CD52 antibody | YTH34.5





Rat anti Human CD52:FITC
Rat anti Human CD52:Low Endotoxin
Rat anti Human CD52
Rat anti Human CD52:RPE
Rat anti Human CD52:Amethyst Orange
- Product Type
- Monoclonal Antibody
- Clone
- YTH34.5
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1642FT | F | 25 µg |
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MCA1642F | F | 0.1 mg |
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MCA1642EL | C CT E F IP P RE WB | 0.5 mg |
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MCA1642T | C F P RE | 20 µg |
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MCA1642 | C CT E F IP P RE WB | 0.2 mg |
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MCA1642PE | F | 100 Tests |
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MCA1642PET | F | 25 Tests/0.25ml |
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MCA1642AMO | F | 0.1 mg |
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The apparent molecular mass of the native antigen on SDS-PAGE is 25-29 kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998).
CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Humanized versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilizedPack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- Pack Size: 100 Tests
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose Pack Size: 25 Tests/0.25ml0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Pack Size: 25 µgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P31358
- Entrez Gene
- CD52
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005624 membrane fraction
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0031225 anchored to membrane
- GO:0045730 respiratory burst
- Regulatory
- For research purposes only
Applications of CD52 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Cytotoxic Assays | 50ug/ml (Use human serum as complement source) | ||
ELISA | |||
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunohistology - Resin | |||
Immunoprecipitation | |||
Western Blotting | |||
Cytotoxic Assays | 50ug/ml (Use human serum as complement source) | ||
ELISA | |||
Flow Cytometry | 1/20 | ||
Immunohistology - Frozen | 1/20 | ||
Immunohistology - Paraffin | 1/20 | 1/20 Pack Size: 20 µg 1/40 Pack Size: 0.2 mg |
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Immunohistology - Resin | |||
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat | 1/2 Pack Size: 100 Tests Neat Pack Size: 25 Tests/0.25ml |
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Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Histology Positive Control Tissue
- Tonsil
- Histology Positive Control Tissue
- Tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2b Negative Control:FITC | MCA6006F | F | 0.1 mg |
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Rat IgG2b Negative Control:Low Endotoxin | MCA6006EL | F | 0.5 mg |
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Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
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Rat IgG2b Negative Control:RPE | MCA6006PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
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Human anti Alemtuzumab | HCA175 | E | 0.1 mg |
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Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD52 antibody
-
Klangsinsirikul, P. et al. (2002) Campath-1G causes rapid depletion of circulating host dendritic cells (DCs) before allogeneic transplantation but does not delay donor DC reconstitution.
Blood. 99: 2586-91. -
Ratzinger, G. et al. (2003) Differential CD52 expression by distinct myeloid dendritic cell subsets: implications for alemtuzumab activity at the level of antigen presentation in allogeneic graft-host interactions in transplantation.
Blood. 101: 1422-9. -
Zand, M.S. et al. (2005) A renewable source of donor cells for repetitive monitoring of T- and B-cell alloreactivity.
Am J Transplant. 5: 76-86. -
Westermann, J et al. (2005) CD52 Is Not a Promising Immunotherapy Target for Most Patients with Multiple Myeloma
International Journal of Hematology. 82 (3): 248-50. -
Gopcsa, L. et al. (2005) Extensive flow cytometric characterization of plasmacytoid dendritic cell leukemia cells.
Eur J Haematol. 75: 346-51. -
Rodig SJ et al. (2006) Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1H).
Clin Cancer Res. 12 (23): 7174-9. -
Golay, J. et al. (2006) The sensitivity of acute lymphoblastic leukemia cells carrying the t(12;21) translocation to campath-1H-mediated cell lysis.
Haematologica. 91: 322-30. -
Miles, R.R. et al. (2007) Immunophenotypic identification of possible therapeutic targets in paediatric non-Hodgkin lymphomas: a children's oncology group report.
Br J Haematol. 138: 506-12. -
Chang, S.T. et al. (2007) CD52 expression in non-mycotic T- and NK/T-cell lymphomas.
Leuk Lymphoma. 48: 117-21. -
Piccaluga, P.P. et al. (2007) Expression of CD52 in peripheral T-cell lymphoma.
Haematologica. 92: 566-7. -
Reimer, P. et al. (2009) Autologous stem-cell transplantation as first-line therapy in peripheral T-cell lymphomas: results of a prospective multicenter study.
J Clin Oncol. 27: 106-13. -
Hu, Y. et al. (2009) Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model.
Immunology. 128: 260-70. -
Rizzo, K. et al. (2009) Novel CD19 expression in a peripheral T cell lymphoma: A flow cytometry case report with morphologic correlation.
Cytometry B Clin Cytom. 76: 142-9. -
Haniffa, M. et al. (2009) Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation.
J Exp Med. 206: 371-85. -
Bisig, B. et al. (2013) CD30-positive peripheral T-cell lymphomas share molecular and phenotypic features.
Haematologica. 98 (8): 1250-8. -
Paulus, A. et al. (2015) Immunophenotyping of Waldenströms macroglobulinemia cell lines reveals distinct patterns of surface antigen expression: potential biological and therapeutic implications.
PLoS One. 10 (4): e0122338. -
Hotta, R. et al. (2016) CD52-Negative NK Cells Are Abundant in the Liver and Less Susceptible to Alemtuzumab Treatment.
PLoS One. 11 (8): e0161618. -
Buckstein, R. et al. (2016) Alemtuzumab and CHOP Chemotherapy for the Treatment of Aggressive Histology Peripheral T Cell Lymphomas: A Multi-Center Phase I Study.
Clin Lymphoma Myeloma Leuk. 16 (1): 18-28.e4. -
Craig, J.W. et al. (2018) Assessment of CD52 expression in "double-hit" and "double-expressor" lymphomas: Implications for clinical trial eligibility.
PLoS One. 13 (7): e0199708.
Further Reading
-
Salisbury JR et al. (1994) Immunohistochemical analysis of CDw52 antigen expression in non-Hodgkin's lymphomas.
J Clin Pathol. 47 (4): 313-7. -
Hale G et al. (1998) Improving the outcome of bone marrow transplantation by using CD52 monoclonal antibodies to prevent graft-versus-host disease and graft rejection.
Blood. 92 (12): 4581-90.
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