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CD52 antibody | YTH34.5

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Anti Human CD52 Antibody, clone YTH34.5 thumbnail image 1 Anti Human CD52 Antibody, clone YTH34.5 thumbnail image 2 Anti Human CD52 Antibody, clone YTH34.5 thumbnail image 3 Anti Human CD52 Antibody, clone YTH34.5 thumbnail image 4 Anti Human CD52 Antibody, clone YTH34.5 thumbnail image 5
Anti Human CD52 Antibody, clone YTH34.5 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Rat IgG2b isotype control (MCA6006AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A700) and Amethyst Orange conjugated Rat anti Human CD52 (MCA1642AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD52 Antibody, clone YTH34.5 gallery image 2
Figure A. Pacific Blue conjugated mouse anti human CD3 (MCA463PB) and RPE conjugated rat IgG2b isotype control (MCA6006PE). Figure B. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB) and RPE conjugated mouse anti human CD52 (MCA1642PE). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD52 Antibody, clone YTH34.5 gallery image 3
Published customer image:
Rat anti Human CD52 antibody, clone YTH34.5 (MCA1642) used for staining lymphoma tissue specimens by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Positive CD52 IHC from representative DHL cases reveals a range of staining intensities: 1+ (A), 2+ (B), and 3+ (C). H&E stain of the strongly CD52-positive DHL case depicted in C (D). Many cases exhibited uniform staining intensity (E), while others showed variable CD52 expression (F).

From: Craig JW, Mina MJ, Crombie JL, LaCasce AS, Weinstock DM, Pinkus GS, et al. (2018)
Assessment of CD52 expression in "double-hit" and "double-expressor" lymphomas: Implications for clinical trial eligibility.
PLoS ONE 13(7): e0199708.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD52 Antibody, clone YTH34.5 gallery image 4
Published customer image:
FITC conjugated Mouse anti Human CD52 antibody, clone YTH34.5 (MCA1642F) used for the evaluation of CD52 expression on human cell lines by flow cytometry.
Image caption:
WM-specific antigen expression compared across RPCI-WM1, BCWM.1 and MWCL-1 cell lines.
Fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antibodies were used as presented above. All cell lines were negative for CD10 and 11c. Blue line indicates RPCI-WM1 antigen expression, red line indicates MWCL-1 antigen expression and green line indicates antigen expression in BCWM.1 cell line. Only BCWM.1 an MWCL-1 were CD19+, CD20+ and FMC7+. Expression of CD138 and κ-light-chain was seen only on MWCL-1 and RPCI-WM1 cells. CD28 expression was markedly more observable on RPCI-WM1 tumor cells as compared to MWCL-1 or BCWM.1.

From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)
Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.
PLoS ONE 10(4): e0122338.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD52 Antibody, clone YTH34.5 gallery image 5
Published customer image:
FITC conjugated Mouse anti Human CD52 antibody, clone YTH34.5 (MCA1642F) used for the evaluation of CD52 expression on RPCI-WM1 cells by flow cytometry.
Image caption:
Expression of WM-specific antigens present on RPCI-WM1.
For antigen detection, fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antigen-specific antibodies were used. Flow cytometry shows the RPCI-WM1 cell line to be CD10, 11c, 19, 20, 23, 27, 40, 52 and 79b negative and CD22, 28, 38, 45, 45RO, 38, 138, κ light chain and intracellular-κ positive.

From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)
Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.
PLoS ONE 10(4): e0122338.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti Human CD52:FITC

Rat anti Human CD52:Low Endotoxin

Rat anti Human CD52

Rat anti Human CD52:RPE

Rat anti Human CD52:Amethyst Orange

Product Type
Monoclonal Antibody
Clone
YTH34.5
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1642F
Datasheet
F
SDS
0.1 mg loader

MCA1642FT
Datasheet
F
SDS
25 µg loader

MCA1642EL
Datasheet
C CT E F IP P RE WB
SDS
0.5 mg loader

MCA1642
Datasheet
C CT E F IP P RE WB
SDS
0.2 mg loader

MCA1642T
Datasheet
C F P RE
SDS
20 µg loader

MCA1642PET
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1642PE
Datasheet
F
SDS
100 Tests loader

MCA1642AMO
Datasheet
F
SDS
0.1 mg loader

Rat anti Human CD52 antibody, clone YTH34.5 recognizes the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link (Xia et al. 1991).

The apparent molecular mass of the native antigen on SDS-PAGE is 25-29 kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998).

CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Humanized versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
Pack Size: 25 Tests/0.25ml
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Pack Size: 100 Tests
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P31358
Entrez Gene
CD52
GO Terms
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0031225 anchored to membrane
GO:0045730 respiratory burst
Regulatory
For research purposes only

Applications of CD52 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Cytotoxic Assays 50ug/ml (Use human serum as complement source)
ELISA
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting
Cytotoxic Assays 50ug/ml (Use human serum as complement source)
ELISA
Flow Cytometry 1/20
Immunohistology - Frozen 1/20
Immunohistology - Paraffin 1/20 1/40 Pack Size: 0.2 mg
1/20 Pack Size: 20 µg
Immunohistology - Resin
Immunoprecipitation
Western Blotting
Flow Cytometry Neat Neat Pack Size: 25 Tests/0.25ml
1/2 Pack Size: 100 Tests
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil
Histology Positive Control Tissue
Tonsil
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2b Negative Control:FITC MCA6006F F 0.1 mg loader
Rat IgG2b Negative Control:Low Endotoxin MCA6006EL F 0.5 mg loader
Rat IgG2b Negative Control MCA6006GA F 0.1 mg loader
Rat IgG2b Negative Control:RPE MCA6006PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human anti Alemtuzumab HCA174 E 0.1 mg loader
Human anti Alemtuzumab HCA175 E 0.1 mg loader
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for CD52 antibody

  1. Klangsinsirikul, P. et al. (2002) Campath-1G causes rapid depletion of circulating host dendritic cells (DCs) before allogeneic transplantation but does not delay donor DC reconstitution.
    Blood. 99: 2586-91.
  2. Ratzinger, G. et al. (2003) Differential CD52 expression by distinct myeloid dendritic cell subsets: implications for alemtuzumab activity at the level of antigen presentation in allogeneic graft-host interactions in transplantation.
    Blood. 101: 1422-9.
  3. Zand, M.S. et al. (2005) A renewable source of donor cells for repetitive monitoring of T- and B-cell alloreactivity.
    Am J Transplant. 5: 76-86.
  4. Westermann, J et al. (2005) CD52 Is Not a Promising Immunotherapy Target for Most Patients with Multiple Myeloma
    International Journal of Hematology. 82 (3): 248-50.
  5. Gopcsa, L. et al. (2005) Extensive flow cytometric characterization of plasmacytoid dendritic cell leukemia cells.
    Eur J Haematol. 75: 346-51.
  6. Rodig SJ et al. (2006) Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1H).
    Clin Cancer Res. 12 (23): 7174-9.
  7. Golay, J. et al. (2006) The sensitivity of acute lymphoblastic leukemia cells carrying the t(12;21) translocation to campath-1H-mediated cell lysis.
    Haematologica. 91: 322-30.
  8. Miles, R.R. et al. (2007) Immunophenotypic identification of possible therapeutic targets in paediatric non-Hodgkin lymphomas: a children's oncology group report.
    Br J Haematol. 138: 506-12.
  9. Chang, S.T. et al. (2007) CD52 expression in non-mycotic T- and NK/T-cell lymphomas.
    Leuk Lymphoma. 48: 117-21.
  10. Piccaluga, P.P. et al. (2007) Expression of CD52 in peripheral T-cell lymphoma.
    Haematologica. 92: 566-7.
  11. Reimer, P. et al. (2009) Autologous stem-cell transplantation as first-line therapy in peripheral T-cell lymphomas: results of a prospective multicenter study.
    J Clin Oncol. 27: 106-13.
  12. Hu, Y. et al. (2009) Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model.
    Immunology. 128: 260-70.
  13. Rizzo, K. et al. (2009) Novel CD19 expression in a peripheral T cell lymphoma: A flow cytometry case report with morphologic correlation.
    Cytometry B Clin Cytom. 76: 142-9.
  14. Haniffa, M. et al. (2009) Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation.
    J Exp Med. 206: 371-85.
  15. Bisig, B. et al. (2013) CD30-positive peripheral T-cell lymphomas share molecular and phenotypic features.
    Haematologica. 98 (8): 1250-8.
  16. Paulus, A. et al. (2015) Immunophenotyping of Waldenströms macroglobulinemia cell lines reveals distinct patterns of surface antigen expression: potential biological and therapeutic implications.
    PLoS One. 10 (4): e0122338.
  17. Hotta, R. et al. (2016) CD52-Negative NK Cells Are Abundant in the Liver and Less Susceptible to Alemtuzumab Treatment.
    PLoS One. 11 (8): e0161618.
  18. Buckstein, R. et al. (2016) Alemtuzumab and CHOP Chemotherapy for the Treatment of Aggressive Histology Peripheral T Cell Lymphomas: A Multi-Center Phase I Study.
    Clin Lymphoma Myeloma Leuk. 16 (1): 18-28.e4.
  19. Craig, J.W. et al. (2018) Assessment of CD52 expression in "double-hit" and "double-expressor" lymphomas: Implications for clinical trial eligibility.
    PLoS One. 13 (7): e0199708.

Further Reading

  1. Salisbury JR et al. (1994) Immunohistochemical analysis of CDw52 antigen expression in non-Hodgkin's lymphomas.
    J Clin Pathol. 47 (4): 313-7.
  2. Hale G et al. (1998) Improving the outcome of bone marrow transplantation by using CD52 monoclonal antibodies to prevent graft-versus-host disease and graft rejection.
    Blood. 92 (12): 4581-90.

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