CD49f antibody | NKI-GoH3

Rat anti Human CD49f

Product Type
Monoclonal Antibody
Clone
NKI-GoH3
Isotype
IgG2a
Specificity
CD49f

Product Code Applications Pack Size List Price Your Price Qty
MCA699GA
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SDS Safety Datasheet SDS
C F IF IP 0.1 mg loader
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loader
MCA699T
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SDS Safety Datasheet SDS
C F IF IP 25 µg loader
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MCA699
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C F IF IP 0.2 mg loader
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Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49f, also known as the VLA-6 alpha chain. CD49f is a 1107 amino acid ~120 kDa cell surface glycoprotein that forms distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex expressed on various human epithelial cells.

Rat anti Human CD49f antibody, clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common acute lymphoblastic leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin.

Rat anti Human CD49f antibody, clone NKI-GoH3 blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog
Pig
Cynomolgus monkey
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
BALB/c mouse mammary tumor cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 platelets in 100ul.
Histology Positive Control Tissue
Human Tonsil

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References for CD49f antibody

  1. Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055.
    Oxford University Press.
  2. Dangerfield, J. et al. (2002) PECAM-1 (CD31) homophilic interaction up-regulates alpha6beta1 on transmigrated neutrophils in vivo and plays a functional role in the ability of alpha6 integrins to mediate leukocyte migration through the perivascular basement membrane.
    J Exp Med. 196: 1201-11.
  3. Alais, S. et al. (2001) HEMCAM/CD146 downregulates cell surface expression of beta1 integrins.
    J Cell Sci. 114: 1847-59.
  4. da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
    Am J Pathol. 177: 1534-48.
  5. Jensen, K.B. et al. (2010) Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis.
    Nat Protoc. 5 (5): 898-911.
  6. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens.
    Edited by Knapp, W. et al. Oxford University Press p1029-1032.
  7. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
    J Histochem Cytochem. 34 (8): 1037-46.
  8. Sonnenberg, A. et al. (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody.
    J Biol Chem. 262 (21): 10376-83.
  9. View The Latest Product References
  10. Hemler, M.E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures.
    J Biol Chem. 263 (16): 7660-5.
  11. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
    Vet Immunol Immunopathol. 53 (3-4): 329-34.
  12. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6.
    Nature. 336 (6198): 487-9.
  13. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8.
    J Cell Biol. 110 (6): 2145-55.
  14. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  15. Sonnenberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties.
    J Cell Sci. 96 ( Pt 2): 207-17.
  16. Sonnenberg, A. et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic.
    J Biol Chem. 263 (28): 14030-8.
  17. Le Bellego, F. et al. (2005) Cytoskeleton reorganization mediates alpha6beta1 integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells.
    Reprod Biol Endocrinol. 3: 19.
  18. Anderson, C. et al. (2009) Sonic hedgehog-dependent synthesis of laminin alpha1 controls basement membrane assembly in the myotome.
    Development. 136: 3495-504.
  19. Collins, C.A. et al. (2011) Reprogramming adult dermis to a neonatal state through epidermal activation of β-catenin
    Development.138: 5189-99.
  20. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Veterinary Parasitology. 15 Feb [Epub ahead of print]
  21. Mastrogiannaki M et al. (2016) β-catenin stabilization in skin fibroblasts causes fibrotic lesions by preventing adipocyte differentiation of the reticular dermis.
    J Invest Dermatol. pii: S0022-202X(16)00489-9. [Epub ahead of print]
  22. Schäfer, G. et al. (2013) The role of inflammation in HPV infection of the Oesophagus.
    BMC Cancer. 13: 185.
  23. Peuhu. E, et al. (2017) Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice
    PLOS ONE. 12 (10): e0186628.
  24. Rayagiri, S.S. et al. (2018) Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
    Nat Commun. 9 (1): 1075.
  25. Loureiro, J. et al. (2019) Conjugation of the T1 sequence from CCN1 to fibrin hydrogels for therapeutic vascularization.
    Mater Sci Eng C Mater Biol Appl. 104: 109847.
  26. Ikeda, A. et al. (2020) Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells.
    Sci Rep. 10 (1): 19959.
  27. Haining, E.J. et al. (2017) Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.
    Platelets. 28 (7): 629-42.
  28. Lorenzo-Martín, L.F. et al. (2022) The Rho guanosine nucleotide exchange factors Vav2 and Vav3 modulate epidermal stem cell function.
    Oncogene. May 09 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Functional Assays

Immunofluorescence

Synonyms
Integrin Alpha 6 Chain
VLA-6
RRID
AB_321462
UniProt
P23229
Entrez Gene
ITGA6
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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