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CD4 antibody | RPA-T4

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Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 1 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 2 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 3 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 4 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 5 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 6 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 7 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 8 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 9 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 10 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 11 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 12 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 13 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 14 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 15 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 16 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 17 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 18 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 19 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 20 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 21 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 22 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 23 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 24 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 25 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 26 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 27 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 28 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 29 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 30 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 31 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 32 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 33 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 34 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 35 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 36 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 37 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 38 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 39 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 40 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 41 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 42 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 43 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 44 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 45 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 46 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 47 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 48 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 49 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 50 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 51 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 52 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 53 Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 54
Anti Human CD4 Antibody, clone RPA-T4 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse anti Human CD4 (MCA1267AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD4 Antibody, clone RPA-T4 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and Alexa Fluor 700 conjugated mouse IgG1 isotype control (MCA928A700). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and Alexa Fluor 700 conjugated Mouse anti Human CD4 (MCA1267A700). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD4 Antibody, clone RPA-T4 gallery image 3
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD4 Antibody, clone RPA-T4 gallery image 4
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD4 (MCA1267PE). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD4 Antibody, clone RPA-T4 gallery image 5
Figure A. FITC conjugated mouse anti human CD3 (MCA2184A488F) and Alexa®647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD3 (MCA2184F) and Alexa®647 conjugated mouse anti human CD4 (MCA1267A647). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD4 Antibody, clone RPA-T4 gallery image 6
Figure A. FITC conjugated mouse anti human CD3 (MCA463F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti human CD3 (MCA463F) and RPE conjugated mouse anti human CD4 (MCA1267PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD4 Antibody, clone RPA-T4 gallery image 7
Figure A. RPE conjugated mouse anti human CD3 (MCA463PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti human CD3 (MCA463PE) and FITC conjugated mouse anti human CD4 (MCA1267F). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD4 Antibody, clone RPA-T4 gallery image 8
Figure A. Pacific Blue® conjugated mouse anti human CD19 (MCA1940PB) and Alexa488® conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Pacific Blue® conjugated mouse anti human CD19 (MCA1940PB) and Alexa488® conjugated mouse anti human CD4 (MCA1267A488). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD4 Antibody, clone RPA-T4 gallery image 9
Figure A. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse IgG1 istoype control (MCA928A647). Figure B. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse anti Human CD4 (MCA1267A647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD4 Antibody, clone RPA-T4 gallery image 10
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MCA1267). Low power.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 11
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MCA1267). Medium power.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 12
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MCA1267). Medium power.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 13
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and SBV515 conjugated Mouse anti Human CD4 (MCA1267SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 14
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and SBV515 conjugated Mouse anti Human CD3 (MCA463SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 15
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and SBV515 conjugated Mouse anti Human CD2 (MCA1194SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 16
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MCA1267). High power.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 17
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Blue 700 (STAR210SBB700). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 18
Figure A. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). and StarBright Violet 670 conjugated Mouse anti Human CD2 (MCA1194SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 19
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and StarBright Violet 670 conjugated Mouse anti Human CD3 (MCA463SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 20
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 670 conjugated Mouse anti Human CD4 (MCA1267SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 21
Figure A. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647) and StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 22
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700) and StarBright Violet 440 conjugated Mouse anti Human CD2 (MCA1194SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 23
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 440 conjugated Mouse anti Human CD4 (MCA1267SBV440). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 24
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright Violet 610 conjugated Mouse anti Human CD2 (MCA1194SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 25
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 26
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 610 conjugated Mouse anti Human CD4 (MCA1267SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 27
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 670 (STAR210SBV670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 28
Figure A. PE Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647) and StarBright Violet 570 conjugated Mouse anti Human CD2 (MCA1194SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 29
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 570 conjugated Mouse anti Human CD4 (MCA1267SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 30
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and StarBright Violet 710 conjugated Mouse anti Human CD2 (MCA1194SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 31
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 32
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 790 (STAR210SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 33
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 34
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and StarBright Violet 790 conjugated Mouse anti Human CD38 (MCA1194SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 35
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright Violet 475 conjugated Mouse anti Human CD2 (MCA1194SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 36
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 475 conjugated Mouse anti Human CD4 (MCA1267SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 37
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 475 (STAR210SBV475). All experiments performed on red cell lysed human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 38
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright Violet 475 conjugated Mouse anti Human CD3 (MCA463SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 39
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright UltraViolet 400 conjugated Mouse anti Human CD2 (MCA1194SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 40
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 41
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright UltraViolet 400 conjugated Mouse anti Human CD4 (MCA1267SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 42
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and APC conjugated Mouse IgG2a isotype control (MCA929APC). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and APC conjugated Mouse anti Human CD3 (MCA6146APC). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 43
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790) and FITC conjugated Mouse anti Human CD3 (MCA6146F). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 44
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and PE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and PE conjugated Mouse anti Human CD3 (MCA6146PE). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 45
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and APC conjugated Mouse anti Human CD45RB (MCA1114APC). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 46
Figure A. RPE conjugated Mouse anti Human CD4 (MCA1267PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD4 (MCA1267PE) and FITC conjugated Mouse anti Human CD45RB (MCA1114F). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 47
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and StarBright Violet 760 conjugated Mouse anti Human CD2 (MCA1194SBV760). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 48
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright UltraViolet 665 conjugated Mouse anti Human CD2 (MCA1194SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 49
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV515) and StarBright UltraViolet 665 conjugated Mouse anti Human CD2 (MCA1194SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 50
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 665 (STAR210SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 51
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD4 (MCA1267SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD4 (MCA1267SBV475) and StarBright UltraViolet 665 conjugated Mouse anti Human CD3 (MCA463SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 52
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD3 (MCA463SBV570). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD3 (MCA463SBV570) and StarBright UltraViolet 665 conjugated Mouse anti Human CD4 (MCA1267SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 53
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795) and StarBright UltraViolet 445 conjugated Mouse anti Human CD4 (MCA1267SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD4 Antibody, clone RPA-T4 gallery image 54
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700) and StarBright UltraViolet 445 conjugated Mouse anti Human CD2 (MCA1194SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD4:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
RPA-T4
Isotype
IgG1
Specificity
CD4

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MCA1267A700T
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Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.

Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation
MCA1267A700T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1267A700: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Human PHA blasts
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
Regulatory
For research purposes only
Guarantee
12 months from date of despatch.
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10 Pack Size: 25 Tests/0.25ml
1/2 Pack Size: 100 Tests/1ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 700

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD4 antibody

  1. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
    J Immunol. 167 (8): 4593-9.
  2. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1).
    Blood. 100 (10): 3698-702.
  3. Piatier-Tonneau, D. (1997) CD4 workshop panel report.
    In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.
  4. Pentón-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
    Int Immunopharmacol. 11 (1): 29-38.
  5. Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
    Immunology. 102 (2): 173-9.
  6. Zhang, Y. et al. (2013) Accelerated in vivo. proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
    PLoS Pathog. 9 (4): e1003310.
  7. Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.

Flow Cytometry

RRID
AB_844497
UniProt
P01730
Entrez Gene
CD4
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway

MCA1267A700T

153817

MCA1267A700

150039 155064

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