CD90 antibody | F15-42-1
Mouse anti Human CD90:StarBright Violet 610
- Product Type
- Monoclonal Antibody
- Clone
- F15-42-1
- Isotype
- IgG1
- Specificity
- CD90
Filter by Application:
F Reset| Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a ~25 kDa glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain. Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Purified human brain Thy-1.
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 610 403 607 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat |
- 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
Source Reference
-
McKenzie, J.L. & Fabre, J.W. (1981) Human thy-1: unusual localization and possible functional significance in lymphoid tissues.
J Immunol. 126 (3): 843-50.
References for CD90 antibody
-
Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.
Lancet. 2 (8244): 434-8. -
Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
J Histochem Cytochem. 52 (11): 1495-501. -
Hagood, J.S. et al. (2005) Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis.
Am J Pathol. 167 (2): 365-79. -
Tome, M. et al. (2007) Calponin is expressed by subpopulations of connective tissue cells but not olfactory ensheathing cells in the neonatal olfactory mucosa.
BMC Neurosci. 8: 74. -
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
J Cell Physiol. 214: 75-83. -
Pessina, A. et al. (2010) CD45+/CD133+ positive cells expanded from umbilical cord blood expressing PDX-1 and markers of pluripotency.
Cell Biol Int. 34: 783-90. -
Manochantr, S. et al. (2010) Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells.
J Med Assoc Thai. 93 Suppl 7: S183-91. -
Karlsen, T.A. et al. (2010) Human primary articular chondrocytes, chondroblasts-like cells, and dedifferentiated chondrocytes: differences in gene, microRNA, and protein expression and phenotype.
Tissue Eng Part C Methods. 17: 219-27.
View The Latest Product References
-
Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
Am J Pathol. 177: 2011-21. -
Yin, S. et al. (2010) Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1.
Tissue Eng Part A. 16: 1633-43. -
Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells.
Blood. 116: 3770-9. -
Holzwarth, C. et al. (2010) Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
BMC Cell Biol. 11:11 -
Meng, J. et al (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454. -
Cizeau, J. et al. (2011) Fusogenics: a recombinant immunotoxin-based screening platform to select internalizing tumor-specific antibody fragments.
J Biomol Screen. 16: 90-100. -
Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
J Bone Joint Surg Br. 93: 517-24. -
Shafaei, H. et al. (2011) Effects of human placental serum on proliferation and morphology of human adipose tissue-derived stem cells.
Bone Marrow Transplant. 46: 1464-71. -
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. -
Supokawej, A. et al. (2013) Cardiogenic and myogenic gene expression in mesenchymal stem cells after 5-azacytidine treatment.
Turk J Haematol. 30 (2): 115-21. -
Escobar, C.H. & Chaparro, O. (2016) Xeno-Free Extraction, Culture, and Cryopreservation of Human Adipose-Derived Mesenchymal Stem Cells.
Stem Cells Transl Med. 5 (3): 358-65. -
Shinoda, K. et al. (2016) Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.
Proc Natl Acad Sci U S A. 113 (20): E2842-51. -
Kamprom, W. et al. (2016) Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells.
Stem Cells Int. 2016: 2514326. -
Vaquero, J. et al. (2016) An approach to personalized cell therapy in chronic complete paraplegia: The Puerta de Hierro phase I/II clinical trial.
Cytotherapy. 18 (8): 1025-36. -
Zhang, X. et al. (2017) Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
PLoS One. 12 (6): e0180138. -
Garikipati, V. N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244. -
Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
Stem Cells Int. 2018: 6901761. -
Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 9 (1): 5430. -
Song, H. et al. (2019) MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury.
J Mol Histol. 50 (4): 355-67. -
Paiboon, N. et al. (2019) Gestational Tissue-Derived Human Mesenchymal Stem Cells Use Distinct Combinations of Bioactive Molecules to Suppress the Proliferation of Human Hepatoblastoma and Colorectal Cancer Cells.
Stem Cells Int. 2019: 9748795. -
Sanjurjo-Rodriguez, C. et al. (2020) Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction.
Front Bioeng Biotechnol. 8: 579751. -
Fujii-Tezuka, R. et al. (2021) Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
Regen Ther. 16: 1-11. -
Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
Sci Rep. 12 (1): 682. -
Sirithammajak, S. et al. (2022) Human Mesenchymal Stem Cells Derived from the Placenta and Chorion Suppress the Proliferation while Enhancing the Migration of Human Breast Cancer Cells.
Stem Cells Int. 2022: 4020845. -
Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
Int J Mol Sci. 23 (24): 16211. -
Kruchen, A. et al. (2023) Epigenetic Modification of Mesenchymal Stromal Cells Derived from Bone Marrow and Embryonal Tumors to Facilitate Immunotherapeutic Approaches in Pediatric Malignancies.
Curr Issues Mol Biol. 45 (3): 2121-35. -
Payet, M. et al. (2023) Inflammatory Mesenchymal Stem Cells Express Abundant Membrane-Bound and Soluble Forms of C-Type Lectin-like CD248.
Int J Mol Sci. 24 (11): 9546. -
Tiraihi, T. et al. (2023) A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue
Cell Tissue Biol. 17 (6): 639-52. -
Tripathy, N.K. et al. (2018) Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells.
J Stem Cells Regen Med. 14 (1): 27-33. -
Altaie, A. et al. (2022) Device-Based Enrichment of Knee Joint Synovial Cells to Drive MSC Chondrogenesis Without Prior Culture Expansion In Vitro: A Step Closer to 1-Stage Orthopaedic Procedures.
Am J Sports Med. 50 (1): 152-161. -
Uthanaphun, T. et al. (2024) PL-hMSC and CH-hMSC derived soluble factors inhibit proliferation but improve hGBM cell migration by activating TGF-β and inhibiting Wnt signaling.
Biosci Rep.44(5):BSR20231964. [Epub ahead of print]. -
Park, C.Y. et al. (2025) External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force.
Biomater Res. 29: 0265. -
Campbell, T.M. et al. (2025) Capsular stem cell function and tissue composition are associated with symptoms and radiographic severity in people with knee osteoarthritis.
Ther Adv Musculoskelet Dis. 17: 1759720X251321941.
- Synonyms
- THY1
- RRID
- AB_2943404
- UniProt
- P04216
- Entrez Gene
- THY1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005783 endoplasmic reticulum
- GO:0005100 Rho GTPase activator activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0030336 negative regulation of cell migration
- View More GO Terms
- GO:0030426 growth cone
- GO:0034235 GPI anchor binding
- GO:0043547 positive regulation of GTPase activity
- GO:0045121 membrane raft
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
MCA90SBV610
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up