CD33 antibody | WM53
Mouse anti Human CD33:StarBright Violet 475
- Product Type
- Monoclonal Antibody
- Clone
- WM53
- Isotype
- IgG1
- Specificity
- CD33
Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin. Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to StarBright Violet 475 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Human AML cells
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 475 405 479 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
DO NOT FREEZE.
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
Source Reference
-
Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).
Dis Markers. 5 (4): 215-25.
References for CD33 antibody
-
Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
Br J Haematol. 69 (2): 163-71. -
Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.
Immunol Cell Biol. 71:571-81. -
Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.
Exp Gerontol. 35: 213-24. -
Vamvakopoulos, J. et al. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
Eur J Immunol. 32 (10): 2988-96. -
Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.
BMC Cardiovasc Disord. 3: 6. -
Dahl C et al. (2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
Allergy. 59 (10): 1087-96. -
Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.
Eur J Immunol. 35: 243-51. -
Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
Blood. 106 (10): 3567-74.
View The Latest Product References
-
Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
J Leukoc Biol. 79: 46-58. -
Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.
Leuk Res. 31: 211-20. -
Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.
Anal Chem. 84 (20): 8830-6. -
McCormack E et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
Blood. 121 (7): e34-42. -
Hu, Z. et al. (2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody
J Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53. -
Hernández-Caselles T et al. (2019) CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019: 6032141.
- UniProt
- P20138
- Entrez Gene
- CD33
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007267 cell-cell signaling
- GO:0008285 negative regulation of cell proliferation
MCA1271SBV475
100005851If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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