CD235a antibody | YTH89.1
Rat anti Human CD235a
- Product Type
- Monoclonal Antibody
- Clone
- YTH89.1
- Isotype
- IgG2b
- Specificity
- CD235a
Antibody Quality and Performance Guarantee
| Rat anti Human CD235a antibody, clone YTH89.1 recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Cytotoxic Assays |  |
||
| Flow Cytometry | |
1/10 | 1/25 |
| Immunohistology - Frozen | |
1/100 | 1/1000 |
| Immunohistology - Paraffin | |
1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human hypophysis
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
References for CD235a antibody
-
Outram, S. et al. (1988) Erythromyeloid lineage fidelity is conserved in erythroleukaemia.
Leuk Res. 12 (8): 651-7. -
Jokiranta, T.S. & Meri, S. (1993) Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement.
J Immunol. 151 (4): 2124-31. -
Hoang, T. et al. (1996) Opposing effects of the basic helix-loop-helix transcription factor SCL on erythroid and monocytic differentiation.
Blood. 87: 102-11. -
Babiker, A.A. et al. (2002) Transfer of prostasomal CD59 to CD59-deficient red blood cells results in protection against complement-mediated hemolysis.
Am J Reprod Immunol. 47 (3): 183-92. -
Lahlil, R. et al. (2004) SCL assembles a multifactorial complex that determines glycophorin A expression.
Mol Cell Biol. 24: 1439-52. -
Challier, J.C. et al. (2005) Immunocytological evidence for hematopoiesis in the early human placenta.
Placenta. 26: 282-8. -
Huang, Y.C. et al. (2009) Oral small-molecule tyrosine kinase inhibitor midostaurin (PKC412) inhibits growth and induces megakaryocytic differentiation in human leukemia cells.
Toxicol In Vitro. 23: 979-85. -
Tiziani, S. et al. (2009) Metabolomic profiling of drug responses in acute myeloid leukaemia cell lines.
PLoS One. 2009;4(1):e4251.
View The Latest Product References
-
Basu, S.
(2010) Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.
Hematology. 15: 236-9. -
Saha, S. et al. (2011) Elevated levels of redox regulators, membrane-bound globin chains, and cytoskeletal protein fragments in hereditary spherocytosis erythrocyte proteome.
Eur J Haematol. 87: 259-66. -
Lucky AB et al. (2016) Plasmodium knowlesi Skeleton-Binding Protein 1 Localizes to the 'Sinton and Mulligan' Stipplings in the Cytoplasm of Monkey and Human Erythrocytes.
PLoS One. 11 (10): e0164272. -
Scanlon, V.M. et al. (2022) Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation.
Sci Rep. 12 (1): 16218.
- Synonyms
- Glycophorin A
- RRID
- AB_323506
- UniProt
- P02724
- Entrez Gene
- GYPA
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
MCA506G
153698
162173
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