CD235a antibody | YTH89.1

Rat anti Human CD235a

Product Type
Monoclonal Antibody
Clone
YTH89.1
Isotype
IgG2b
Specificity
CD235a

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MCA506G
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C CT F P 0.2 mg loader
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Rat anti Human CD235a antibody, clone YTH89.1 recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Cytotoxic Assays
Flow Cytometry 1/10 1/25
Immunohistology - Frozen 1/100 1/1000
Immunohistology - Paraffin 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human hypophysis

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References for CD235a antibody

  1. Outram, S. et al. (1988) Erythromyeloid lineage fidelity is conserved in erythroleukaemia.
    Leuk Res. 12 (8): 651-7.
  2. Jokiranta, T.S. & Meri, S. (1993) Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement.
    J Immunol. 151 (4): 2124-31.
  3. Basu, S. (2010) Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.
    Hematology. 15: 236-9.
  4. Hoang, T. et al. (1996) Opposing effects of the basic helix-loop-helix transcription factor SCL on erythroid and monocytic differentiation.
    Blood. 87: 102-11.
  5. Lahlil, R. et al. (2004) SCL assembles a multifactorial complex that determines glycophorin A expression.
    Mol Cell Biol. 24: 1439-52.
  6. Tiziani, S. et al. (2009) Metabolomic profiling of drug responses in acute myeloid leukaemia cell lines.
    PLoS One. 2009;4(1):e4251.
  7. Saha, S. et al. (2011) Elevated levels of redox regulators, membrane-bound globin chains, and cytoskeletal protein fragments in hereditary spherocytosis erythrocyte proteome.
    Eur J Haematol. 87: 259-66.
  8. Challier, J.C. et al. (2005) Immunocytological evidence for hematopoiesis in the early human placenta.
    Placenta. 26: 282-8.
  9. View The Latest Product References
  10. Huang, Y.C. et al. (2009) Oral small-molecule tyrosine kinase inhibitor midostaurin (PKC412) inhibits growth and induces megakaryocytic differentiation in human leukemia cells.
    Toxicol In Vitro. 23: 979-85.
  11. Lucky AB et al. (2016) Plasmodium knowlesi Skeleton-Binding Protein 1 Localizes to the 'Sinton and Mulligan' Stipplings in the Cytoplasm of Monkey and Human Erythrocytes.
    PLoS One. 11 (10): e0164272.
  12. Babiker, A.A. et al. (2002) Transfer of prostasomal CD59 to CD59-deficient red blood cells results in protection against complement-mediated hemolysis.
    Am J Reprod Immunol. 47 (3): 183-92.

Flow Cytometry

Synonyms
Glycophorin A
RRID
AB_323506
UniProt
P02724
Entrez Gene
GYPA
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0042802 identical protein binding
GO:0044419 interspecies interaction between organisms
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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