CD195 antibody | HEK/1/85a

Rat anti Human CD195

Product Type
Monoclonal Antibody
Clone
HEK/1/85a
Isotype
IgG2a
Specificity
CD195

Product Code Applications Pack Size List Price Your Price Qty
MCA2175
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
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loader
MCA2175GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5.

CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO cells transfected with human CCR5
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cell of the rat Y3 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul fresh whole blood.

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References for CD195 antibody

  1. Mueller, A. et al. (2002) Pathways for internalization and recycling of the chemokine receptor CCR5.
    Blood 99 (3): 785-91.
  2. Pilch-Cooper, H.A. et al. (2011) Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5.
    Blood 118: 1015-9.
  3. Ramirez, R. et al. (2006) Microinflammation in hemodialysis is related to a preactivated subset of monocytes.
    Hemodial Int. 10 Suppl 1:S24-7.
  4. Liptrott, N.J. et al. (2009) The impact of cytokines on the expression of drug transporters, cytochrome P450 enzymes and chemokine receptors in human PBMC.
    Br J Pharmacol. 156: 497-508.
  5. Bönsch, C. et al. (2015) Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
    PLoS One 10 (4): e0125396.
  6. Holmannova, D. et al. (2017) Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
    Perfusion. 32 (2): 141-50.

Synonyms
CCR5
RRID
AB_324334
UniProt
P51681
Entrez Gene
CCR5
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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