CD195 antibody | HEK/1/85a
Rat anti Human CD195 antibody, clone HEK/1/85a (MCA2175) used for the immunoprecipitation of CD195 from transfected CHO lysates.
Image caption:
Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.
From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10(4): e0125396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD195 antibody, clone HEK/1/85a (MCA2175GA) used for the detection of CD195 expression by transfected CHO cells by immunofluorescence.
Image caption:
Spatial and temporal resolution of arrestin2-CCR5 association. A CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with chemokine analogs (100 nM) as indicated and the redistribution of arrestin2-GFP was followed by live fluorescence microscopy. Images captured prior to (0 min) and after ligand treatment (6 min) are shown. B CHO-CCR5 cells stably transfected with arrestin2-GFP (green), preincubated with rhodamine-labeled anti-CCR5 antibody (red), were washed and then incubated 90 min at 37°C with chemokine analogs (100 nM) prior to image capture. Maximal intensity projections are shown. Scale bar = 20 μm.
From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
PLoS ONE 10(4): e0125396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IP IF ResetRat anti Human CD195
- Product Type
- Monoclonal Antibody
- Clone
- HEK/1/85a
- Isotype
- IgG2a
- Specificity
- CD195
Antibody Quality and 
Unrivaled 
| Rat anti Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5. CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CHO cells transfected with human CCR5
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cell of the rat Y3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul fresh whole blood.
References for CD195 antibody
-
Mueller, A. et al. (2002) Pathways for internalization and recycling of the chemokine receptor CCR5.
Blood 99 (3): 785-91. -
Pilch-Cooper, H.A. et al. (2011) Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5.
Blood 118: 1015-9. -
Ramirez, R. et al. (2006) Microinflammation in hemodialysis is related to a preactivated subset of monocytes.
Hemodial Int. 10 Suppl 1:S24-7. -
Liptrott, N.J. et al. (2009) The impact of cytokines on the expression of drug transporters, cytochrome P450 enzymes and chemokine receptors in human PBMC.
Br J Pharmacol. 156: 497-508. -
Bönsch, C. et al. (2015) Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
PLoS One 10 (4): e0125396. -
Holmannova, D. et al. (2017) Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
Perfusion. 32 (2): 141-50.
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