CD16 antibody | YFC120.5
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F ResetRat anti Human CD16
- Product Type
- Monoclonal Antibody
- Clone
- YFC120.5
- Isotype
- IgG2b
- Specificity
- CD16
Antibody Quality and Performance Guarantee
| Rat anti Human CD16 antibody, clone YFC120.5 binds specifically to the leucocyte low affinity Fc receptor (Fc RIII), also known as CD16. The antigen is expressed by neutrophils, NK cells, K cells and a subpopulation of macrophages. It recognizes a ~50-70 kDa leukocyte surface antigen. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human neutrophils.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from Lou rat fused with the rat myeloma cell line. Y3/Ag.1.2.3.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
|
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| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
References for CD16 antibody
-
Huizinga, T.W. et al. (1990) Maternal genomic neutrophil FcRIII deficiency leading to neonatal isoimmune neutropenia.
Blood. 76: 1927-32. -
Vermeulen, B.L. et al. (2012) Natural killer cells: frequency, phenotype and function in healthy cats.
Vet Immunol Immunopathol. 150: 69-78. -
Drescher, B. et al. (2003) Glycosylation of FcgammaRIII in N163 as mechanism of regulating receptor affinity.
Immunology. 110: 335-40. -
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164: 46-59. -
Orr, C.F. et al. (2005) A possible role for humoral immunity in the pathogenesis of Parkinson's disease.
Brain. 128 (Pt 11): 2665-74. -
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164 (1-2): 46-59.
- Synonyms
- FcRIII
- RRID
- AB_2104004
- UniProt
- O75015
- P08637
- Entrez Gene
- FCGR3B
- FCGR3A
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
MCA617G
161368
1806
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