CD16 antibody | YFC120.5

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Anti Human CD16 Antibody, clone YFC120.5 gallery image 1 — Staining of human peripheral blood lymphocytes with Rat anti Human CD16 (**MCA617G**).

Rat anti Human CD16

Product Type: Monoclonal Antibody
Clone: YFC120.5
Isotype: IgG2b

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA617G Datasheet	C F SDS	0.2 mg	Log in		Get Quote

Rat anti Human CD16 antibody, clone YFC120.5 binds specifically to the leucocyte low affinity Fc receptor (Fc RIII), also known as CD16. The antigen is expressed by neutrophils, NK cells, K cells and a subpopulation of macrophages. It recognizes a ~50-70 kDa leukocyte surface antigen.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Cat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Human neutrophils.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from Lou rat fused with the rat myeloma cell line. Y3/Ag.1.2.3.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: O75015; P08637
Entrez Gene: FCGR3B; FCGR3A
GO Terms: GO:0005886 plasma membrane; GO:0005576 extracellular region; GO:0004872 receptor activity; GO:0006955 immune response; GO:0031225 anchored to membrane; GO:0019864 IgG binding; GO:0016021 integral to membrane; GO:0050776 regulation of immune response
Regulatory: For research purposes only

Applications of CD16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	C E P WB	1 ml	Log in	Get Quote
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	C E IF P WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	C E P RE	1 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	F	0.5 ml	Log in	Get Quote
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)	STAR71D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	F	0.5 ml	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rat IgG2b Negative Control	MCA6006GA	F	0.1 mg	Log in		Get Quote

Application Based External Images

Flow Cytometry

Product Specific References

References for CD16 antibody

Huizinga, T.W. et al. (1990) Maternal genomic neutrophil FcRIII deficiency leading to neonatal isoimmune neutropenia.
Blood. 76: 1927-32.
Vermeulen, B.L. et al. (2012) Natural killer cells: frequency, phenotype and function in healthy cats.
Vet Immunol Immunopathol. 150: 69-78.
Drescher, B. et al. (2003) Glycosylation of FcgammaRIII in N163 as mechanism of regulating receptor affinity.
Immunology. 110: 335-40.
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164: 46-59.
Orr, C.F. et al. (2005) A possible role for humoral immunity in the pathogenesis of Parkinson's disease.
Brain. 128 (Pt 11): 2665-74.
Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
Vet Microbiol. 164 (1-2): 46-59.

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