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CD16 antibody | YFC120.5

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Anti Human CD16 Antibody, clone YFC120.5 gallery image 1
Staining of human peripheral blood lymphocytes with Rat anti Human CD16 (MCA617G).

Rat anti Human CD16

Product Type
Monoclonal Antibody
Clone
YFC120.5
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA617G
Datasheet
C F
SDS
0.2 mg loader

Rat anti Human CD16 antibody, clone YFC120.5 binds specifically to the leucocyte low affinity Fc receptor (Fc RIII), also known as CD16. The antigen is expressed by neutrophils, NK cells, K cells and a subpopulation of macrophages. It recognizes a ~50-70 kDa leukocyte surface antigen.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human neutrophils.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from Lou rat fused with the rat myeloma cell line. Y3/Ag.1.2.3.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O75015
P08637
Entrez Gene
FCGR3B
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2b Negative Control MCA6006GA F 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD16 antibody

  1. Huizinga, T.W. et al. (1990) Maternal genomic neutrophil FcRIII deficiency leading to neonatal isoimmune neutropenia.
    Blood. 76: 1927-32.
  2. Vermeulen, B.L. et al. (2012) Natural killer cells: frequency, phenotype and function in healthy cats.
    Vet Immunol Immunopathol. 150: 69-78.
  3. Drescher, B. et al. (2003) Glycosylation of FcgammaRIII in N163 as mechanism of regulating receptor affinity.
    Immunology. 110: 335-40.
  4. Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
    Vet Microbiol. 164: 46-59.
  5. Orr, C.F. et al. (2005) A possible role for humoral immunity in the pathogenesis of Parkinson's disease.
    Brain. 128 (Pt 11): 2665-74.
  6. Vermeulen, B.L. et al. (2013) Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus.
    Vet Microbiol. 164 (1-2): 46-59.

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