CD137 antibody | JG1.6A

Rat anti Human CD137

Product Type
Monoclonal Antibody
Clone
JG1.6A
Isotype
IgG2a
Specificity
CD137

Product Code Applications Pack Size List Price Your Price Qty
MCA2653
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 0.2 mg loader
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loader
MCA2653T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Human CD137 antibody, clone JG1.6A recognizes human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and regulates T-cell activation and proliferation.

Rat anti Human CD137 antibody, clone JG1.6A induces γ-interferon activity in isolated T-cells as measured by ELISA (Rajendran et al. 2019).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human CD137-Fc.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Lymph node cells from immunised Lou rat were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Further Reading

  1. Pollok, K.E. et al. (1993) Inducible T cell antigen 4-1BB. Analysis of expression and function.
    J Immunol. 150 (3): 771-81.
  2. Alderson, M.R. et al. (1994) Molecular and biological characterization of human 4-1BB and its ligand.
    Eur J Immunol. 24 (9): 2219-27.
  3. Schwarz, H. et al. (1997) CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies.
    Biochem Biophys Res Commun. 235 (3): 699-703.
  4. Cannons, J.L. et al. (2001) 4-1BB ligand induces cell division, sustains survival, and enhances effector function of CD4 and CD8 T cells with similar efficacy.
    J Immunol. 167 (3): 1313-24.

Synonyms
4-1BB Ligand Receptor
RRID
AB_844478
UniProt
Q07011
Entrez Gene
TNFRSF9
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0008285 negative regulation of cell proliferation
View more products with CD137 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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