Rat anti Human CD137 antibody, clone JG1.6A recognizes human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and is reported to regulate T-cell activation and proliferation.
Rat anti Human CD137 antibody, clone JG1.6A has been reported for use in functional studies.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- Recombinant human CD137-Fc.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised Lou rat were fused with cells of the mouse NS-1 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
induction of apoptosis
negative regulation of cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD137 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
Pollok, K.E. et al. (1993) Inducible T cell antigen 4-1BB. Analysis of expression and function.
J Immunol. 150 (3): 771-81.
Alderson, M.R. et al. (1994) Molecular and biological characterization of human 4-1BB and its ligand.
Eur J Immunol. 24 (9): 2219-27.
Schwarz, H. et al. (1997) CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies.
Biochem Biophys Res Commun. 235 (3): 699-703.
Cannons, J.L. et al. (2001) 4-1BB ligand induces cell division, sustains survival, and enhances effector function of CD4 and CD8 T cells with similar efficacy.
J Immunol. 167 (3): 1313-24.
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