CD137 antibody | JG1.6A

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Rat anti Human CD137

Product Type
Monoclonal Antibody
Clone
JG1.6A
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2653T E F 25 µg
MCA2653 E F 0.2 mg
Rat anti Human CD137 antibody, clone JG1.6A recognizes human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and is reported to regulate T-cell activation and proliferation.

Rat anti Human CD137 antibody, clone JG1.6A has been reported for use in functional studies.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Pack Size: 25 µg
Phosphate buffered saline
Pack Size: 0.2 mg
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Recombinant human CD137-Fc.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Lymph node cells from immunised Lou rat were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q07011 Related reagents
Entrez Gene
TNFRSF9 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0008285 negative regulation of cell proliferation
Regulatory
For research purposes only

Applications of CD137 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

Further Reading

  1. Pollok, K.E. et al. (1993) Inducible T cell antigen 4-1BB. Analysis of expression and function.
    J Immunol. 150 (3): 771-81.
  2. Alderson, M.R. et al. (1994) Molecular and biological characterization of human 4-1BB and its ligand.
    Eur J Immunol. 24 (9): 2219-27.
  3. Schwarz, H. et al. (1997) CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies.
    Biochem Biophys Res Commun. 235 (3): 699-703.
  4. Cannons, J.L. et al. (2001) 4-1BB ligand induces cell division, sustains survival, and enhances effector function of CD4 and CD8 T cells with similar efficacy.
    J Immunol. 167 (3): 1313-24.