Cartilage Oligomeric Matrix Protein antibody | MA37C94 (HC484D1)

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Rat anti Human Cartilage Oligomeric Matrix Protein

Product Type
Monoclonal Antibody
Clone
MA37C94 (HC484D1)
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1455G C E IP P WB 0.2 mg
Rat anti Human cartilage oligomeric matrix protein antibody, clone MA37C94 recognizes human cartilage oligomeric matrix protein (COMP), otherwise known as thrombospondin-5 (TSP-5). COMP is a 757 amino acid matrix glycoprotein bearing four EGF-like domains, a single TSP C-terminal domain and eight TSP type-3 repeats (Uniprot P49747). Defects in the COMP gene can lead to the presence of pseudoachondroplasia or multiple epiphyseal dysplasia (Posey et al. 2014).

Rat anti Human cartilage oligomeric matrix protein, clone MA37C94 recognizes an epitope located in the central portion of the molecule and has been described as suitable for use in western blotting (Gagarina et al. 2008) and immunohistochemistry (Grigoriadis et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human cartilage derived COMP
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Wistar rats were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P49747 Related reagents
Entrez Gene
COMP Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0006915 apoptosis
GO:0007155 cell adhesion
GO:0005201 extracellular matrix structural constituent
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0043395 heparan sulfate proteoglycan binding
GO:0060173 limb development
Regulatory
For research purposes only

Applications of Cartilage Oligomeric Matrix Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/200 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Application Based External Images

Immunocytochemistry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Cartilage Oligomeric Matrix Protein antibody

  1. Grigoriadis, A.et al. (2006) Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data.
    Breast Cancer Res. 8: R56.
  2. Milz, S. et al. (2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.
    J Anat. 211: 1-7.
  3. Gagarina, V. et al. (2008) Cartilage oligomeric matrix protein protects cells against death by elevating members of the IAP family of survival proteins.
    J Biol Chem 283: 648-59.
  4. Jäger, M. et al. (2006) Ovine cord blood accommodates multipotent mesenchymal progenitor cells.
    In Vivo. 20: 205-14.
  5. Kobayashi, M. et al. (2016) Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin.
    J Invest Dermatol. Mar 8. pii: S0022-202X(16)30856-9. [Epub ahead of print]
  6. Milz, S. et al. (2005) An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings.
    J Anat. 206 (1): 37-45.
  7. Zilkens, C. et al. (2010) Spinning around or stagnation - what do osteoblasts and chondroblasts really like?
    Eur J Med Res. 15 (1): 35-43.
  8. Milz, S. et al. (2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.
    J Anat. 211 (1): 1-7.
  9. Inui, S. et al. (2011) Identification and characterization of cartilage oligomeric matrix protein as a novel pathogenic factor in keloids.
    Am J Pathol. 179 (4): 1951-60.
  10. Viehöfer, A.F. et al. (2015) The molecular composition of the extracellular matrix of the human iliolumbar ligament.
    Spine J. 15 (6): 1325-31.
  11. Nemoto, M. et al. (2013) Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.
    J Equine Sci. 24 (2): 17-24.