Cartilage Oligomeric Matrix Protein antibody | MA37C94 (HC484D1)
Rat anti Human cartilage oligomeric matrix protein antibody, clone MA37C94 (MCA1455G) used for the staining of invasive ductal carcinoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded material following heat mediated antigen retrieval in citrate buffer, pH6.0.
Image caption:
Immunohistochemical analysis of periostin (POSTN), IL8 and cartilage oligomeric matrix protein (COMP). (a) POSTN-positive invasive ductal carcinoma (IDC; ×400), in which both epithelial and stromal cells show cytoplasmic expression. (b) POSTN-negative IDC in which only the spindle shaped stromal cells are stained (×400). (c) IL8 (×100), showing positive staining only in the malignant breast epithelial cells. (d) COMP expression in the epithelial and stromal cells of an IDC, showing strong expression in both stromal and epithelial cells (×100).
From: Grigoriadis, A. et al. 2006
Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data.
Breast Cancer Res. 2006;8(5):R56.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
WB P ResetRat anti Human Cartilage Oligomeric Matrix Protein
- Product Type
- Monoclonal Antibody
- Clone
- MA37C94 (HC484D1)
- Isotype
- IgG2a
- Specificity
- Cartilage Oligomeric Matrix Protein
Antibody Quality and Performance Guarantee
| Rat anti Human cartilage oligomeric matrix protein antibody, clone MA37C94 recognizes human cartilage oligomeric matrix protein (COMP), otherwise known as thrombospondin-5 (TSP-5). COMP is a 757 amino acid matrix glycoprotein bearing four EGF-like domains, a single TSP C-terminal domain and eight TSP type-3 repeats (Uniprot P49747). Defects in the COMP gene can lead to the presence of pseudoachondroplasia or multiple epiphyseal dysplasia (Posey et al. 2014). Rat anti Human cartilage oligomeric matrix protein, clone MA37C94 recognizes an epitope located in the central portion of the molecule and has been described as suitable for use in western blotting (Gagarina et al. 2008) and immunohistochemistry (Grigoriadis et al. 2006). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human cartilage derived COMP
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Wistar rats were fused with cells of the mouse NS1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
||
| Western Blotting | |
1/200 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Cartilage Oligomeric Matrix Protein antibody
-
Grigoriadis, A.et al. (2006) Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data.
Breast Cancer Res. 8: R56. -
Milz, S. et al. (2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.
J Anat. 211: 1-7. -
Gagarina, V. et al. (2008) Cartilage oligomeric matrix protein protects cells against death by elevating members of the IAP family of survival proteins.
J Biol Chem 283: 648-59. -
Jäger, M. et al. (2006) Ovine cord blood accommodates multipotent mesenchymal progenitor cells.
In Vivo. 20: 205-14. -
Kobayashi, M. et al. (2016) Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin.
J Invest Dermatol. 136 (6): 1143-9. -
Milz, S. et al. (2005) An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings.
J Anat. 206 (1): 37-45. -
Zilkens, C. et al. (2010) Spinning around or stagnation - what do osteoblasts and chondroblasts really like?
Eur J Med Res. 15 (1): 35-43. -
Milz, S. et al. (2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.
J Anat. 211 (1): 1-7.
View The Latest Product References
-
Inui, S. et al. (2011) Identification and characterization of cartilage oligomeric matrix protein as a novel pathogenic factor in keloids.
Am J Pathol. 179 (4): 1951-60. -
Viehöfer, A.F. et al. (2015) The molecular composition of the extracellular matrix of the human iliolumbar ligament.
Spine J. 15 (6): 1325-31. -
Nemoto, M. et al. (2013) Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.
J Equine Sci. 24 (2): 17-24. -
Gong, S.D. et al. (2019) Elevated plasma cartilage oligomeric matrix protein (COMP) level are associated with the progression of non-traumatic osteonecrosis of femoral head.
Clin Chim Acta. 490: 214-21.
- Synonyms
- COMP
- UniProt
- P49747
- Entrez Gene
- COMP
- GO Terms
- GO:0005509 calcium ion binding
- GO:0006915 apoptosis
- GO:0007155 cell adhesion
- GO:0005201 extracellular matrix structural constituent
- GO:0005518 collagen binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0008201 heparin binding
- View More GO Terms
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0060173 limb development
MCA1455G
151417
164872
1702
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