C3d antibody | 3

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Rat anti Human C3d

Product Type
Monoclonal Antibody
Clone
3
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6161GA
Datasheet
E IP
SDS
0.1 mg loader

Rat anti Human C3d antibody, clone 3 (also known as clone YB2/39-11-7), recognizes a linear antigen on C3d. This antibody also detects C3, C3b, iC3b and C3dg, it does not recognize C3c. To distinguish C3 from C3b, a Mouse anti Human C3a/C3a desArg antibody (MCA6163GA) can be used.

C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3, resulting in its cleavage to anaphylatoxin C3a and opsonin C3b. Once formed, C3b targets microbial cell surface receptors, marking them for phagocytosis by macrophages. The activity of C3b is heavily regulated to prevent accidental host tissue damage. Of these regulators, circulating Factor I, in the presence of co-factors, cleaves C3b to generate inactivated C3b (iC3b). iC3b’s inactive designation relates to its inability to assist in the formation of convertases. In the presence of membrane bound complement receptor 1, iC3b is cleaved further to C3dg, before further cleavage into C3d and C3c. C3d is able to interact with complement receptor 2, which is expressed on the surface of B cells. C3d is involved in pathogen opsonisation, using a thiolester bond to covalently coat pathogens (Ricklin et al. 2016).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
Regulatory
For research purposes only

Applications of C3d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

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