C3c antibody | 4

Rat anti Human C3c

Product Type
Monoclonal Antibody
Clone
4
Isotype
IgG2a
Specificity
C3c

Product Code Applications Pack Size List Price Your Price Qty
MCA6158GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP 0.1 mg loader
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loader
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Rat anti Human C3c antibody, clone 4, recognizes the cleaved human C3 fragment; C3c also recognizes C3b and iC3b. C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3, resulting in its cleavage to anaphylatoxin C3a and opsonin C3b. Once formed, C3b targets microbial cell surface receptors, marking them for phagocytosis by macrophages. The activity of C3b is heavily regulated to prevent accidental host tissue damage. Of these regulators, circulating Factor I, in the presence of co-factors, cleaves C3b to generate inactivated C3b (iC3b). This inactive designation relates to its inability to assist in the formation of convertases. In the presence of membrane bound complement receptor 1, iC3b is then cleaved further to C3dg, before further cleavage into C3d and C3c (Ricklin et al. 2016). The exact role of C3c is yet to be fully defined.

With C5a being highly active with a half-life less than 5 mins, the various C3 activation products are more readily detectable in assays, as they remain for a few hours (Bosmann et al. 2012). C3c is considered a valuable marker for inflammatory processes since it is more stable and develops out of C3 within one hour at body temperature. As such C3c is often chosen as an indicator of complement activation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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