Rat anti Human C3c antibody, clone 4,
recognizes the cleaved human C3 fragment; C3c also recognizes C3b and iC3b. C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3, resulting in its cleavage to anaphylatoxin C3a and opsonin C3b. Once formed, C3b targets microbial cell surface receptors, marking them for phagocytosis by macrophages. The activity of C3b is heavily regulated to prevent accidental host tissue damage. Of these regulators, circulating Factor I, in the presence of co-factors, cleaves C3b to generate inactivated C3b (iC3b). This inactive designation relates to its inability to assist in the formation of convertases. In the presence of membrane bound complement receptor 1, iC3b is then cleaved further to C3dg, before further cleavage into C3d and C3c (Ricklin et al. 2016
). The exact role of C3c is yet to be fully defined.
With C5a being highly active with a half-life less than 5 mins, the various C3 activation products are more readily detectable in assays, as they remain for a few hours (Bosmann et al. 2012
). C3c is considered a valuable marker for inflammatory processes since it is more stable and develops out of C3 within one hour at body temperature. As such C3c is often chosen as an indicator of complement activation.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of C3c antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.