C3c antibody | 4
|Rat anti Human C3c antibody, clone 4, recognizes the cleaved human C3 fragment; C3c also recognizes C3b and iC3b. C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3, resulting in its cleavage to anaphylatoxin C3a and opsonin C3b. Once formed, C3b targets microbial cell surface receptors, marking them for phagocytosis by macrophages. The activity of C3b is heavily regulated to prevent accidental host tissue damage. Of these regulators, circulating Factor I, in the presence of co-factors, cleaves C3b to generate inactivated C3b (iC3b). This inactive designation relates to its inability to assist in the formation of convertases. In the presence of membrane bound complement receptor 1, iC3b is then cleaved further to C3dg, before further cleavage into C3d and C3c (Ricklin et al. 2016). The exact role of C3c is yet to be fully defined.
With C5a being highly active with a half-life less than 5 mins, the various C3 activation products are more readily detectable in assays, as they remain for a few hours (Bosmann et al. 2012). C3c is considered a valuable marker for inflammatory processes since it is more stable and develops out of C3 within one hour at body temperature. As such C3c is often chosen as an indicator of complement activation.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Entrez Gene
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
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