BUB3 antibody | AB03/4E7-5

Mouse anti BUB3

Product Type
PrecisionAb Monoclonal
Clone
AB03/4E7-5
Isotype
IgG2b
Format
Purified
Specificity
BUB3

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VMA00785
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Mouse anti BUB3 antibody recognizes mitotic checkpoint protein BUB3.

BUB3 is a constitutively-expressed protein that acts as a mitotic checkpoint protein in the nucleus (Ersvær et al. 2020). BUB3 is one of the core components of the mitotic surveillance mechanism spindle assembly cell cycle checkpoint (SAC), alongside BUB1. This checkpoint prevents onset of anaphase until all chromosomes have been correctly attached to microtubules during the cell cycle (Li et al. 2018). Cells lacking BUB3 have an increased rate of chromosome mis-segregation, leading to aneuploidy, and furthermore to cell cycle delay or death (Yang and Lacefield 2016). Dysregulation of mitotic checkpoint genes can lead to cancer, and cytoplasmic expression of BUB3 has been found to be significantly correlated with recurrence. BUB3 has been suggested as a prognostic marker in prostate cancer (Ersvær et al. 2020).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse BUB3 antibody detects a band of approximately 38 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-328 of human BUB3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
O43684
Entrez Gene
BUB3
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0005634 nucleus
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0007126 meiosis
GO:0008608 attachment of spindle microtubules to kinetochore
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0051301 cell division
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
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