BUB3 antibody | AB03/4E7-5
Mouse anti BUB3 antibody recognizes mitotic checkpoint protein BUB3.
BUB3 is a constitutively-expressed protein that acts as a mitotic checkpoint protein in the nucleus (Ersvær et al. 2020). BUB3 is one of the core components of the mitotic surveillance mechanism spindle assembly cell cycle checkpoint (SAC), alongside BUB1. This checkpoint prevents onset of anaphase until all chromosomes have been correctly attached to microtubules during the cell cycle (Li et al. 2018). Cells lacking BUB3 have an increased rate of chromosome mis-segregation, leading to aneuploidy, and furthermore to cell cycle delay or death (Yang and Lacefield 2016). Dysregulation of mitotic checkpoint genes can lead to cancer, and cytoplasmic expression of BUB3 has been found to be significantly correlated with recurrence. BUB3 has been suggested as a prognostic marker in prostate cancer (Ersvær et al. 2020).
BUB3 is a constitutively-expressed protein that acts as a mitotic checkpoint protein in the nucleus (Ersvær et al. 2020). BUB3 is one of the core components of the mitotic surveillance mechanism spindle assembly cell cycle checkpoint (SAC), alongside BUB1. This checkpoint prevents onset of anaphase until all chromosomes have been correctly attached to microtubules during the cell cycle (Li et al. 2018). Cells lacking BUB3 have an increased rate of chromosome mis-segregation, leading to aneuploidy, and furthermore to cell cycle delay or death (Yang and Lacefield 2016). Dysregulation of mitotic checkpoint genes can lead to cancer, and cytoplasmic expression of BUB3 has been found to be significantly correlated with recurrence. BUB3 has been suggested as a prognostic marker in prostate cancer (Ersvær et al. 2020).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse BUB3 antibody detects a band of approximately 38 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-328 of human BUB3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43684
- Entrez Gene
- BUB3
- GO Terms
- GO:0005515 protein binding
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0007126 meiosis
- GO:0008608 attachment of spindle microtubules to kinetochore
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0051301 cell division
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of BUB3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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