BUB3 antibody | AB03/4E7-5

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Anti BUB3 Antibody, clone AB03/4E7-5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti BUB3 Antibody, clone AB03/4E7-5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti BUB3 Antibody, clone AB03/4E7-5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with BUB3 antibody (1/1000, **VMA00785**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to BUB3 (molecular weight 38 kDa)

Anti BUB3 Antibody, clone AB03/4E7-5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of BUB3 from Jurkat whole cell lysate. The Input lane is 10% input; the BUB3-IP lane is the immunoprecipitation with the BUB3 antibody (2.5 µg, **VMA00785**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA691). Western blot analysis was performed using BUB3 antibody (1/1000, **VMA00785**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to BUB3.

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WB IP

Mouse anti BUB3

Product Type: PrecisionAb Monoclonal
Clone: AB03/4E7-5
Isotype: IgG2b
Format: Purified
Specificity: BUB3

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00785 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti BUB3 antibody recognizes mitotic checkpoint protein BUB3.

BUB3 is a constitutively-expressed protein that acts as a mitotic checkpoint protein in the nucleus (Ersvær et al. 2020). BUB3 is one of the core components of the mitotic surveillance mechanism spindle assembly cell cycle checkpoint (SAC), alongside BUB1. This checkpoint prevents onset of anaphase until all chromosomes have been correctly attached to microtubules during the cell cycle (Li et al. 2018). Cells lacking BUB3 have an increased rate of chromosome mis-segregation, leading to aneuploidy, and furthermore to cell cycle delay or death (Yang and Lacefield 2016). Dysregulation of mitotic checkpoint genes can lead to cancer, and cytoplasmic expression of BUB3 has been found to be significantly correlated with recurrence. BUB3 has been suggested as a prognostic marker in prostate cancer (Ersvær et al. 2020).

Target Species: Human
Western Blotting: Mouse BUB3 antibody detects a band of approximately 38 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-328 of human BUB3
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O43684
Entrez Gene: BUB3
GO Terms: GO:0005515 protein binding; GO:0000236 mitotic prometaphase; GO:0000777 condensed chromosome kinetochore; GO:0005829 cytosol; GO:0005634 nucleus; GO:0007094 mitotic cell cycle spindle assembly checkpoint; GO:0007126 meiosis; GO:0008608 attachment of spindle microtubules to kinetochore; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; View More GO Terms; GO:0051301 cell division; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle