BUB3 antibody | AB03/4E7-5
IP-MS Validated
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WB IP ResetMouse anti BUB3
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB03/4E7-5
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- BUB3
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| Mouse anti BUB3 antibody recognizes mitotic checkpoint protein BUB3. BUB3 is a constitutively-expressed protein that acts as a mitotic checkpoint protein in the nucleus (Ersvær et al. 2020). BUB3 is one of the core components of the mitotic surveillance mechanism spindle assembly cell cycle checkpoint (SAC), alongside BUB1. This checkpoint prevents onset of anaphase until all chromosomes have been correctly attached to microtubules during the cell cycle (Li et al. 2018). Cells lacking BUB3 have an increased rate of chromosome mis-segregation, leading to aneuploidy, and furthermore to cell cycle delay or death (Yang and Lacefield 2016). Dysregulation of mitotic checkpoint genes can lead to cancer, and cytoplasmic expression of BUB3 has been found to be significantly correlated with recurrence. BUB3 has been suggested as a prognostic marker in prostate cancer (Ersvær et al. 2020). |
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- Target Species
- Human
- Western Blotting
- Mouse BUB3 antibody detects a band of approximately 38 kDa in HEK293 cell lysates
- Species Cross-Reactivity
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Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-328 of human BUB3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- O43684
- Entrez Gene
- BUB3
- GO Terms
- GO:0005515 protein binding
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0007126 meiosis
- GO:0008608 attachment of spindle microtubules to kinetochore
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- View More GO Terms
- GO:0051301 cell division
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
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