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Dupilumab antibody | AbD48542

Human anti Dupilumab

Product Type
Monoclonal Antibody
Clone
AbD48542
Isotype
Fab antibody
Specificity
Dupilumab

Product Code Applications Pack Size List Price Your Price Qty
TZA017
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Dupilumab Antibody, clone AbD48542 is a paratope specific, non-inhibitory anti-idiotypic antibody (Type 2) that specifically recognizes the free human monoclonal antibody drug dupilumab, and dupilumab in complex with IL-4Rα, but not free IL-4Rα. This antibody can be used to measure total dupilumab levels (free as well as in complex with human IL-4Rα) in serum from patients.

This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA017) and bivalent Fab conjugated to HRP (TZA017P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free dupilumab captured via immobilized IL-4Rα.
TZA017 can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Dupilumab (Dupixent) is a human IgG4/kappa antibody inhibiting both IL‐4 and IL-13 receptors, thereby blocking these receptor signaling pathways. Dupilumab inhibits the signaling of interleukins (IL) that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis.

View a summary of all anti-dupilumab antibodies
20th Anniversary

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Dupilumab
Affinity
The monovalent intrinsic affinity of AbD48542ad was measured as KD = 179 nM by real time, label free molecular interaction analysis on immobilized dupilumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a registered trademark of Regeneron Pharmaceuticals, Sanofi-Aventis.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD48542ad can be used as a detection antibody, together with TZA016 as the capture antibody to detect dupilumab total drug levels in the serum of patients in PK Bridging ELISA.
Note that both TZA016 (capture) and TZA017 (detection) contain the same peptide tags, therefore using anti-tag antibodies for detecting TZA017 is not possible.

Protocol: PK Bridging ELISA to measure total drug

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Dupilumab HCA383 E 0.1 mg loader
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Description Human anti Dupilumab
Human anti Dupilumab HCA384 E 0.1 mg loader
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Description Human anti Dupilumab
Human anti Dupilumab TZA014 E 0.1 mg loader
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Description Human anti Dupilumab
Human anti Dupilumab TZA015 E 0.1 mg loader
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Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA015P E 0.1 mg loader
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Description Human anti Dupilumab:HRP
Human anti Dupilumab TZA016 E 0.1 mg loader
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Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA017P E 0.1 mg loader
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Description Human anti Dupilumab:HRP
BiSpyCatcher2:HRP TZC002P IY 1 mg loader
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Description BiSpyCatcher2:HRP

Synonyms
Dupixent

TZA017

164129

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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