Dupilumab antibody | AbD48542
Human anti Dupilumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD48542
- Isotype
- Fab antibody
- Specificity
- Dupilumab
Human Anti-Dupilumab Antibody, clone AbD48542 is a paratope specific, non-inhibitory anti-idiotypic antibody (Type 2) that specifically recognizes the free human monoclonal antibody drug dupilumab, and dupilumab in complex with IL-4Rα, but not free IL-4Rα. This antibody can be used to measure total dupilumab levels (free as well as in complex with human IL-4Rα) in serum from patients. This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA017) and bivalent Fab conjugated to HRP (TZA017P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free dupilumab captured via immobilized IL-4Rα. TZA017 can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog. View a summary of all SpyCatcher products Dupilumab (Dupixent) is a human IgG4/kappa antibody inhibiting both IL‐4 and IL-13 receptors, thereby blocking these receptor signaling pathways. Dupilumab inhibits the signaling of interleukins (IL) that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis. View a summary of all anti-dupilumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Dupilumab
- Affinity
- The monovalent intrinsic affinity of AbD48542ad was measured as KD = 179 nM by real time, label free molecular interaction analysis on immobilized dupilumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a registered trademark of Regeneron Pharmaceuticals, Sanofi-Aventis. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD48542ad can be used as a detection antibody, together with TZA016 as the capture antibody to detect dupilumab total drug levels in the serum of patients in PK Bridging ELISA.
Note that both TZA016 (capture) and TZA017 (detection) contain the same peptide tags, therefore using anti-tag antibodies for detecting TZA017 is not possible.
Protocol: PK Bridging ELISA to measure total drug
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
SpyTag3 Peptide | BLP086 | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | SpyTag3 Peptide | ||||||
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Dupilumab | HCA383 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab | ||||||
Human anti Dupilumab | HCA384 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab | ||||||
Human anti Dupilumab | TZA014 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab | ||||||
Human anti Dupilumab | TZA015 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab | ||||||
Human anti Dupilumab:HRP | TZA015P | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab:HRP | ||||||
Human anti Dupilumab | TZA016 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab | ||||||
Human anti Dupilumab:HRP | TZA017P | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Dupilumab:HRP | ||||||
BiSpyCatcher2:HRP | TZC002P | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | BiSpyCatcher2:HRP |
- Synonyms
- Dupixent
TZA017
164129If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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