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Dupilumab antibody | AbD47092

Human anti Dupilumab:HRP

Product Type
Monoclonal Antibody
Clone
AbD47092
Isotype
Fab antibody
Specificity
Dupilumab

Product Code Applications Pack Size List Price Your Price Qty
TZA015P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Dupilumab Antibody, clone AbD47092 is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug dupilumab. TZA015 neither recognizes free IL-4Ra nor dupilumab in complex with IL-4Ra. Consequently, this antibody can be used to measure free dupilumab levels in serum from patients.

This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA015) and bivalent Fab conjugated to HRP (TZA015P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free dupilumab captured via immobilized IL-4Ra.

TZA015 can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Clone AbD47092 is also available in full length IgG1 format under product code HCA384

Dupilumab (Dupixent) is a human IgG4/kappa antibody inhibiting both IL‐4 and IL-13 receptors, thereby blocking these receptor signaling pathways. Dupilumab inhibits the signaling of interleukins (IL) that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis.

View a summary of all anti-dupilumab antibodies

Product Form
A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA015 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Dupilumab
Affinity
The monovalent intrinsic affinity of AbD47092pap was measured as KD = 0.3 nM by real time, label free molecular interaction analysis on immobilized dupilumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a trademark of Regeneron Pharmaceuticals, Sanofi-Aventis

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD47092pap can be used as the detection antibody for dupilumab in a PK bridging ELISA to measure free drug levels in the serum of patients together with TZA014 as the capture reagent.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Dupilumab HCA383 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Dupilumab
Human anti Dupilumab HCA384 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Dupilumab
Human anti Dupilumab TZA014 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab TZA015 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA015P E 0.1 mg
List Price Your Price
Description Human anti Dupilumab:HRP
Human anti Dupilumab TZA016 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab TZA017 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA017P E 0.1 mg
List Price Your Price
Description Human anti Dupilumab:HRP

Synonyms
Dupixent
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

TZA015P

165709

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