Dupilumab antibody | AbD48542
A microtiter plate was coated overnight with Human Anti-Dupilumab Antibody, clone AbD48333ad (TZA016) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of dupilumab. Detection was performed using Human Anti-Dupilumab Antibody, clone AbD48542ad (TZA017) coupled to an HRP-conjugated BiSpyCatcher2 at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human IL-4Ra at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of dupilumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Dupilumab Antibody, clone AbD48542ad (TZA017) or clone AbD47092ad (TZA015), both in the monovalent Fab format, were added. Free dupilumab, still capable of binding to the human IL-4Ra coated plate, was detected using HRP conjugated Mouse anti Human (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, Human Anti-Dupilumab Antibody, clone AbD48542ad (TZA017) at a concentration of 2 μg/ml was added. Detection was performed using an HRP conjugated anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with dupilumab, human IL-4Ra, human IgG1 kappa isotype control or human IgG4Pro control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Dupilumab Antibody, clone AbD48542ad (TZA017) titrated to the given concentrations in PBST. An HRP conjugated anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) was added followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Dupilumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD48542
- Isotype
- Fab antibody
- Specificity
- Dupilumab
Antibody Quality and Performance Guarantee
| Human Anti-Dupilumab Antibody, clone AbD48542 is a paratope specific, non-inhibitory anti-idiotypic antibody (Type 2) that specifically recognizes the free human monoclonal antibody drug dupilumab, and dupilumab in complex with IL-4Rα, but not free IL-4Rα. This antibody can be used to measure total dupilumab levels (free as well as in complex with human IL-4Rα) in serum from patients. This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA017) and bivalent Fab conjugated to HRP (TZA017P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free dupilumab captured via immobilized IL-4Rα. TZA017 can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog. View a summary of all SpyCatcher products Dupilumab (Dupixent) is a human IgG4/kappa antibody inhibiting both IL‐4 and IL-13 receptors, thereby blocking these receptor signaling pathways. Dupilumab inhibits the signaling of interleukins (IL) that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis. View a summary of all anti-dupilumab antibodies |
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- Product Form
- A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA017 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Dupilumab
- Affinity
- The monovalent intrinsic affinity of AbD48542pap was measured as KD = 179 nM by real time, label free molecular interaction analysis on immobilized dupilumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a registered trademark of Regeneron Pharmaceuticals, Sanofi-Aventis. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD48542pap can be used as a detection antibody, together with TZA016 as the capture antibody to detect dupilumab total drug levels in the serum of patients in PK Bridging ELISA.
Protocol: PK Bridging ELISA to measure total drug
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Dupilumab | HCA383 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
| Human anti Dupilumab | HCA384 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
| Human anti Dupilumab | TZA014 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
| Human anti Dupilumab | TZA015 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
| Human anti Dupilumab:HRP | TZA015P | E | 0.1 mg |
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| Description | Human anti Dupilumab:HRP | ||||||
| Human anti Dupilumab | TZA016 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
| Human anti Dupilumab | TZA017 | E | 0.1 mg |
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| Description | Human anti Dupilumab | ||||||
- Synonyms
- Dupixent
TZA017P
165710
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Dupilumab specificity
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