Browsing Modes for Business Users

Dupilumab antibody | AbD48337

Human anti Dupilumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD48337
Isotype
Fab antibody
Specificity
Dupilumab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
New TZA054
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Dupilumab (Drug/Target Complex) Antibody, clone AbD48337 recognizes the monoclonal antibody drug dupilumab when in complex with its target, the Interleukin-4 Receptor alpha (IL-4Rα). It does not recognize free dupilumab or unbound IL-4Rα. It can be used to measure levels of dupilumab in patient samples.

Clone AbD48337 is a monovalent Fab format antibody that can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free dupilumab captured via immobilized IL-4Rα. It is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour.

View a summary of SpyCatcher products

Dupilumab is a fully human monoclonal antibody of the IgG4 subclass that binds to the interleukin-4 subunit α (IL-4Rα) receptor, inhibiting both IL‐4 and IL-13 receptors and blocking the receptor signaling pathways. Dupilumab inhibits the signaling of interleukins that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis.

View a summary of all anti-dupilumab antibodies.

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Dupilumab/IL-4Rα complex
Affinity
The monovalent intrinsic affinity of AbD48337ad was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized IL-4Rα/dupilumab complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a trademark of Regeneron Pharmaceuticals, Sanofi-Aventis.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD48337ad may be used in an indirect ELISA to detect the IL-4Rα/dupilumab complex or to detect dupilumab bound to immobilized IL-4Rα.

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Dupilumab HCA383 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Dupilumab
Human anti Dupilumab HCA384 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Dupilumab
anti SpyTag HCA406 E WB 0.1 mg loader
List Price Your Price
loader
Description anti SpyTag
Human anti Dupilumab TZA014 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab TZA015 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA015P E 0.1 mg
List Price Your Price
Description Human anti Dupilumab:HRP
Human anti Dupilumab TZA016 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab TZA017 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab:HRP TZA017P E 0.1 mg
List Price Your Price
Description Human anti Dupilumab:HRP
BiSpyCatcher2:Biotin TZC002B IY 1 mg
List Price Your Price
Description BiSpyCatcher2:Biotin
BiSpyCatcher2:HRP TZC002P IY 1 mg
List Price Your Price
Description BiSpyCatcher2:HRP

Synonyms
Dupixent
View more products with Dupilumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note