CD44 antibody | YKIX337.8.7
Mouse anti Canine CD44 antibody, clone YKIX337.8.7 (MCA1041GA) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD9, CD90 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
Image caption:
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor
From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.
Mouse anti Canine CD44 antibody, clone YKIX337.8.7 (MCA1041GA) used for the evaluation of CD44 expression on canine bone marrow-derived mesenchymal stem cells by flow cytometry.
Image caption:
The phenotype of cultured bone marrow-derived mesenchymal stem cells (BM-MSCs) from each dog was confirmed before autologous injection.
Expression of CD29, CD34, CD44, CD45, and CD90 by cultured cells was evaluated by flow cytometric analysis using anti-CD29, anti-CD34, anti-CD44, anti-CD45, and anti-CD90 antibodies, after selection of the cell population of interest. Control isotype antibody is plotted in red in each histogram plot. Cultured cells were confirmed as BM-MSCs by their appearance in culture and by the CD29+CD44+CD90+CD34-CD45- phenotype.
From: Muir P, Hans EC, Racette M, Volstad N, Sample SJ, Heaton C, et al. (2016)
Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
PLoS ONE 11(8): e0159095.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Dog CD44 antibody, clone YKIX337.8.7 recognises canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation (Alldinger et al. 2000).
CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 also interacts with other ligands including collagens and metalloproteinases.
Studies have demonstrated that altered CD44 expression is detected in a many forms of invasive and metastatic cancers, CD44 expression has been observed on canine mammary and melanocytic tumors (Serra et al. 2004).
CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 also interacts with other ligands including collagens and metalloproteinases.
Studies have demonstrated that altered CD44 expression is detected in a many forms of invasive and metastatic cancers, CD44 expression has been observed on canine mammary and melanocytic tumors (Serra et al. 2004).
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Raccoon - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Concanavilin A activated Canine T cells.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q28284
- Entrez Gene
- CD44
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005540 hyaluronic acid binding
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD44 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Flow Cytometry | 1/10 | 1/20 | |
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Dog CD34:Alexa Fluor® 647 | MCA2411A647 | F | 100 Tests/1ml |
|
|
| Mouse anti Dog CD34:FITC | MCA2411F | F | 0.1 mg |
|
|
| Mouse anti Dog CD34 | MCA2411GA | F WB | 0.1 mg |
|
|
| Mouse anti Dog CD34:RPE | MCA2411PE | F | 100 Tests |
|
Product Specific References
References for CD44 antibody
-
Cobbold, S. & Metcalfe,S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
Vet Microbiol. 131:237-46. -
Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
Circulation. 104: 2975-80. -
Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24. -
Stein, V.M. et al. (2004) Characterization of canine microglial cells isolated ex vivo.
Vet Immunol Immunopathol. 99: 73-85. -
Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
Vet Immunol Immunopathol. 168 (3-4): 140-6. -
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218. -
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. 50 (6)Oct 08 [Epub ahead of print]. -
Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
PLoS One. 11 (8): e0159095. -
Salinas Tejedor, L. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
Brain Behav Immun. 50: 155-65. -
Heinrich, F. et al. (2015) Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet Immunol Immunopathol. 163 (1-2): 86-92. -
Wijekoon, H.M.S. et al. (2017) Differentiation potential of synoviocytes derived from joints with cranial cruciate ligament rupture and medial patella luxation in dogs.
Res Vet Sci. 114: 370-7. -
Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
Vet Immunol Immunopathol. 202: 130-40. -
Elshafae, S.M. et al. (2017) The Effect of a Histone Deacetylase Inhibitor (AR-42) on Canine Prostate Cancer Growth and Metastasis.
Prostate. 77 (7): 776-93. -
Gouveia, G.M. et al. (2013) Analysis of cancer stem cells in dog’s mammary neoplasias.
Braz J Vet Med, 35(3), 229-35.
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