CD44 antibody | YKIX337.8.7

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Rat anti Dog CD44:Alexa Fluor® 488

Rat anti Dog CD44

Product Type
Monoclonal Antibody
Clone
YKIX337.8.7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1041A488 F 100 Tests/1ml
MCA1041GA C F 0.1 mg
Rat anti Dog CD44 antibody, clone YKIX337.8.7 recognises canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation (Alldinger et al. 2000).

CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 also interacts with other ligands including collagens and metalloproteinases.

Studies have demonstrated that altered CD44 expression is detected in a many forms of invasive and metastatic cancers, CD44 expression has been observed on canine mammary and melanocytic tumors (Serra et al. 2004).

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Raccoon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Concanavilin A activated Canine T cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q28284 Related reagents
Entrez Gene
CD44 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Dog CD34:Alexa Fluor® 647 MCA2411A647 100 Tests/1ml F
Mouse anti Dog CD34:FITC MCA2411F 0.1 mg F
Mouse anti Dog CD34 MCA2411GA 0.1 mg F WB
Mouse anti Dog CD34:RPE MCA2411PE 100 Tests F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD44 antibody

  1. Cobbold, S. & Metcalfe,S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  2. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
    Vet Microbiol. 131:237-46.
  3. Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
    Circulation. 104: 2975-80.
  4. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  5. Stein, V.M. et al. (2004) Characterization of canine microglial cells isolated ex vivo.
    Vet Immunol Immunopathol. 99: 73-85.
  6. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  7. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  8. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. Oct 08 [Epub ahead of print].