CD44 antibody | YKIX337.8.7

Rat anti Dog CD44

Product Type
Monoclonal Antibody
Clone
YKIX337.8.7
Isotype
IgG2a
Specificity
CD44

Product Code Applications Pack Size List Price Your Price Qty
MCA1041GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Dog CD44 antibody, clone YKIX337.8.7 recognizes canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation (Alldinger et al. 2000).

CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 interacts with other ligands including collagens and metalloproteinases.

Altered CD44 expression is detected in many forms of invasive and metastatic cancer, CD44 expression has been observed on canine mammary and melanocytic tumors (Serra et al. 2004).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Raccoon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Concanavilin A activated Canine T cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD44 antibody

  1. Cobbold, S. & Metcalfe,S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  2. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
    Vet Microbiol. 131:237-46.
  3. Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
    Circulation. 104: 2975-80.
  4. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  5. Stein, V.M. et al. (2004) Characterization of canine microglial cells isolated ex vivo.
    Vet Immunol Immunopathol. 99: 73-85.
  6. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  7. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  8. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. 50 (6)Oct 08 [Epub ahead of print].
  9. View The Latest Product References
  10. Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
    PLoS One. 11 (8): e0159095.
  11. Salinas Tejedor, L. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
    Brain Behav Immun. 50: 155-65.
  12. Heinrich, F. et al. (2015) Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
    Vet Immunol Immunopathol. 163 (1-2): 86-92.
  13. Wijekoon, H.M.S. et al. (2017) Differentiation potential of synoviocytes derived from joints with cranial cruciate ligament rupture and medial patella luxation in dogs.
    Res Vet Sci. 114: 370-7.
  14. Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
    Vet Immunol Immunopathol. 202: 130-40.
  15. Elshafae, S.M. et al. (2017) The Effect of a Histone Deacetylase Inhibitor (AR-42) on Canine Prostate Cancer Growth and Metastasis.
    Prostate. 77 (7): 776-93.
  16. Gouveia, G.M. et al. (2013) Analysis of cancer stem cells in dog’s mammary neoplasias.
    Braz J Vet Med, 35(3), 229-35.

Synonyms
H-CAM
PGP-1
UniProt
Q28284
Entrez Gene
CD44
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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