CD34 antibody | 1H6

Mouse anti Dog CD34:RPE

Product Type
Monoclonal Antibody
Clone
1H6
Isotype
IgG1
Specificity
CD34

Product Code Applications Pack Size List Price Your Price Qty
MCA2411PE
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Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells.

Mouse anti dog CD34 antibody, clone 1H6 is a key marker of canine hematopoietic progenitor cells and is reported for use in CD34+ enrichment assays, (Goerner et al. 2001) and (Horn et al. 2004).

Target Species
Dog
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Canine CD34 fusion protein.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
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Description Mouse IgG1 Negative Control:RPE

References for CD34 antibody

  1. McSweeney, P.A. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34.
    Blood. 91 (6): 1977-86.
  2. Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs.
    Blood. 94 (7): 2287-92.
  3. Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells.
    Blood. 95 (2): 581-5.
  4. Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors.
    Blood. 98 (7): 2065-70.
  5. Horn, P.A. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol.
    Blood. 103 (10): 3710-6.
  6. Avallone, G. et al. (2007) The spectrum of canine cutaneous perivascular wall tumors: morphologic, phenotypic and clinical characterization.
    Vet Pathol. 44 (5): 607-20.
  7. Palmieri, C. et al. (2013) Use of electron microscopy to classify canine perivascular wall tumors.
    Vet Pathol. 50 (2): 226-33.
  8. Georges, G. et al. (2001) Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes.
    Blood. 98:3447-55.
  9. View The Latest Product References
  10. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  11. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. Oct 08 [Epub ahead of print].
  12. Lee, S.H. et al. (2016) Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
    Cytotherapy. Oct 28 [Epub ahead of print].
  13. Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
    PLoS One. 11 (8): e0159095.
  14. Rajawat, Y.S. et al. (2021) In Vivo Gene Therapy for Canine SCID-X1 Using Cocal-Pseudotyped Lentiviral Vector.
    Hum Gene Ther. 32 (1-2): 113-27.
  15. Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
    Vet Comp Oncol. 19 (3): 567-77.
  16. Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
    Theriogenology. 169: 1-8.
  17. Jaensch, S. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia
    Australian Veterinary Journal. [Epub ahead of print].

Further Reading

  1. McSweeney, P. et al. (1996) Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein.
    Blood. 88:1992-2003.

RRID
AB_609594
UniProt
Q28270
Entrez Gene
CD34
GO Terms
GO:0016021 integral to membrane
GO:0030246 carbohydrate binding
GO:0016337 cell-cell adhesion
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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