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CD34 antibody | 1H6

Mouse anti Dog CD34:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells.

Mouse anti dog CD34 antibody, clone 1H6 is a key marker of canine hematopoietic progenitor cells and is reported for use in CD34+ enrichment assays, (Goerner et al. 2001) and (Horn et al. 2004).

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Canine CD34 fusion protein.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. McSweeney, P.A. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34.
    Blood. 91 (6): 1977-86.

References for CD34 antibody

  1. Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs.
    Blood. 94 (7): 2287-92.
  2. Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells.
    Blood. 95 (2): 581-5.
  3. Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors.
    Blood. 98 (7): 2065-70.
  4. Georges, G. et al. (2001) Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes.
    Blood. 98:3447-55.
  5. Horn, P.A. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol.
    Blood. 103 (10): 3710-6.
  6. Avallone, G. et al. (2007) The spectrum of canine cutaneous perivascular wall tumors: morphologic, phenotypic and clinical characterization.
    Vet Pathol. 44 (5): 607-20.
  7. Palmieri, C. et al. (2013) Use of electron microscopy to classify canine perivascular wall tumors.
    Vet Pathol. 50 (2): 226-33.
  8. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  9. View The Latest Product References
  10. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. Oct 08 [Epub ahead of print].
  11. Lee, S.H. et al. (2016) Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
    Cytotherapy. Oct 28 [Epub ahead of print].
  12. Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
    PLoS One. 11 (8): e0159095.
  13. Rajawat, Y.S. et al. (2021) In Vivo Gene Therapy for Canine SCID-X1 Using Cocal-Pseudotyped Lentiviral Vector.
    Hum Gene Ther. 32 (1-2): 113-27.
  14. Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
    Vet Comp Oncol. 19 (3): 567-77.
  15. Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
    Theriogenology. 169: 1-8.
  16. Jaensch, S. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia
    Australian Veterinary Journal. [Epub ahead of print].
  17. Salari Sedigh, H. et al. (2023) In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration.
    J Oral Biol Craniofac Res. 13 (3): 403-11.
  18. Papa, P.M. et al. (2023) Intratesticular transplantation of allogenic mesenchymal stem cells mitigates testicular destruction after induced heat stress in Miniature-horse stallions.
    J Equine Vet Sci. 132: 104961.
  19. Rezaei, M. et al. (2019) Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Fibrin Glue for Periodontal Regeneration.
    Int J Periodontics Restorative Dent. 39 (1): e32-e45.
  20. Yang, V.K. et al. (2021) Intravenous administration of allogeneic Wharton jelly-derived mesenchymal stem cells for treatment of dogs with congestive heart failure secondary to myxomatous mitral valve disease.
    Am J Vet Res. 82 (6): 487-93.
  21. Crain, S.K. et al. (2019) Extracellular Vesicles from Wharton's Jelly Mesenchymal Stem Cells Suppress CD4 Expressing T Cells Through Transforming Growth Factor Beta and Adenosine Signaling in a Canine Model.
    Stem Cells Dev. 28 (3): 212-26.
  22. Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
    Vet Comp Oncol. 21 (1): 28-35.
  23. Millanta, F. et al. (2020) Cytologic grading of canine and feline spindle-cell sarcomas of soft tissues and its correlation with histologic grading.
    Top Companion Anim Med. 41: 100458.

Further Reading

  1. McSweeney, P. et al. (1996) Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein.
    Blood. 88:1992-2003.

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0030246 carbohydrate binding
GO:0016337 cell-cell adhesion
GO:0050900 leukocyte migration


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