Denosumab antibody | AbD26296

Human anti Denosumab

Product Type
Monoclonal Antibody
Clone
AbD26296
Isotype
HuCAL Fab monovalent
Specificity
Denosumab

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HCA288
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E 0.1 mg loader
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Human Anti-Denosumab Antibody, clone AbD26296 is a paratope specific, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug denosumab. The antibody does not recognize free RANKL (receptor activator of nuclear factor kappa-B ligand), or denosumab in complex with human RANKL and can be used to measure the levels of free denosumab and biosimilar products in bioanalytical assays.

A pair of anti-denosumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an anti-denosumab antibody in full immunoglobulin format, clone AbD26295_hIgG1 (HCA280) as the detection antibody.

Denosumab (Prolia, Xgeva) is a fully human monoclonal antibody (IgG2/kappa) for the treatment of osteoporosis, treatment-induced bone loss, bone metastases, multiple myeloma, and giant cell tumor of bone. The drug specifically binds to human RANKL, a protein that acts as the primary signal to promote bone removal/resorption and prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors.

View a summary of all anti-denosumab antibodies.

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Denosumab
Affinity
The intrinsic affinity of Human anti Denosumab antibody, clone AbD26296 is KD = 7.1 nM as measured by real time, label free molecular interaction analysis on immobilized Denosumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Prolia and Xgeva are trademarks of Amgen Inc.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Denosumab antibody, clone AbD26296 can be used in a direct or indirect ELISA system or as capture antibody for Denosumab in a bridging ELISA together with HCA280P (AbD26295_hIgG1) as the detection reagent.
Protocol: PK bridging ELISA to measure total drug.

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Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"