Denosumab antibody | AbD26295_hIgG1

Human anti Denosumab

Product Type
Monoclonal Antibody
Clone
AbD26295_hIgG1
Isotype
IgG1
Specificity
Denosumab

Product Code Applications Pack Size List Price Your Price Qty
HCA280
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Human Anti-Denosumab Antibody, clone AbD26295_hIgG1 is a paratope specific anti-idiotypic antibody that specifically recognizes the free human monoclonal antibody denosumab. The antibody does not recognize free RANKL (receptor activator of nuclear factor kappa-B ligand) or denosumab in complex with human RANKL and can be used to measure free denosumab and biosimilar products.

Clone AbD26295_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay.

A pair of anti denosumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD26296 (HCA288) in monovalent Fab format as the capture antibody.

Denosumab (Prolia, Xgeva) is a fully human monoclonal antibody (IgG2/kappa) for the treatment of osteoporosis, treatment-induced bone loss, bone metastases, multiple myeloma, and giant cell tumor of bone. The drug specifically binds to human RANKL a protein that acts as the primary signal to promote bone removal/resorption and prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors.

View a summary of all anti-denosumab antibodies.

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL? phage display library and expressed in a human cell line - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Denosumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=1.8 nM by real time, label-free molecular interaction analysis on immobilized denosumab
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Prolia and Xgeva are registered trademarks of Amgen Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in direct or indirect ELISA and in an ELISA bridging assay as a detection antibody together, when conjugated to HRP, with HCA288 as the capture reagent. It is also suitable as a fully human control or calibrator antibody in an ADA bridging ELISA.
Protocol: PK bridging ELISA to measure free drug
Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Denosumab:HRP HCA280P E 0.1 mg loader
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loader
Description Human anti Denosumab:HRP
Human anti Denosumab HCA281 E 0.1 mg loader
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loader
Description Human anti Denosumab
Human anti Denosumab HCA282 E 0.1 mg loader
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loader
Description Human anti Denosumab
Human anti Denosumab HCA288 E 0.1 mg loader
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loader
Description Human anti Denosumab
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit

Synonyms
Prolia
Xgeva
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
View more products with Denosumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"