MBP antibody | 14

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Rat anti MBP (aa36-50)

Product Type
Monoclonal Antibody
Clone
14
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA408S C* E IF 2 ml
Rat anti MBP antibody, clone 14 recognizes myelin basic protein (MBP), a component of myelin that is beleived to play a role in the myelination of nerves in the central nervous system.

Rat anti MBP antibody, clone 14 has also been reported to work in western blotting (Relucio et al. 2009).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Chicken Expected from Sequence
Horse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Bovine myelin basic protein
Fusion Partners
Spleen cells from immunised outbred rats were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02687 Related reagents
Entrez Gene
MBP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath
Regulatory
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for MBP antibody

  1. Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
    J Neuroimmunol. 12 (4): 253-64.
  2. Glynn, P. et al. (1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.
    J Neurochem. 48 (3): 752-9.
  3. Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
    J Neuroimmunol. 19 (4): 305-15.
  4. Matsuo, A. et al. (1997) Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.
    Am J Pathol. 150: 1253-66.
  5. Jackson, S.J. et al. (2004) Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture.
    Eur J Neurosci. 20: 2267-75.
  6. Friess, M. et al. (2016) Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.
    Cell Calcium. 60 (5): 322-30.
  7. Ou-yang, M.H. et al. (2015) N-terminal region of myelin basic protein reduces fibrillar amyloid-β deposition in Tg-5xFAD mice.
    Neurobiol Aging. 36 (2): 801-11.
  8. Biffi, A. et al. (2006) Gene therapy of metachromatic leukodystrophy reverses neurological damage and deficits in mice.
    J Clin Invest. 116 (11): 3070-82.
  9. Copray, J.C. et al. (2005) p75NTR independent oligodendrocyte death in cuprizone-induced demyelination in C57BL/6 mice.
    Neuropathol Appl Neurobiol. 31 (6): 600-9.
  10. Jagielska, A. et al. (2017) Mechanical Strain Promotes Oligodendrocyte Differentiation by Global Changes of Gene Expression.
    Front Cell Neurosci. 11: 93.