MBP antibody | 14
Rat anti Bovine myelin basic protein antibody, clone 14 (MCA408S) used for the localization of MBP in mouse brain by immunofluorescence on formalin fixed, frozen sections
Image caption:
MBP1-EGFP expression in neuron soma and neurites
Brains of Tg-MBP1-EGFP mice showed globally strong endogenous green fluorescence (E) and MBP1 immunostaining (F) compared to wild-type mice (A, B). MBP1-EGFP expression was detected in the cell bodies and neurites (as designated by the arrows) in the cortex (G) and CA1 of hippocampus (H) but not in the wild-type mice (C, D). MBP1-EGFP co-localized with neuronal NeuN immunolabeling in cortex (I–L) and CA1 region (M–P). Endogenous green fluorescence (I, M), MBP1 immunolabeling (J, N) and NeuN immunolabeling (K, O) were merged in (L) and (P). Scale bars = 1 mm in A, B, E, F and 50 μm in the remaining panels.
From: Ou-Yang MH, Xu F, Liao MC, Davis J, Robinson JK, Van Nostrand WE.
N-terminal region of myelin basic protein reduces fibrillar amyloid-β deposition in Tg-5xFAD mice.
Neurobiol Aging. 2015 Feb;36(2):801-11.
Rat anti myelin basic protein antibody, clone 14 (MCA408S) used to identify myelin basic protein expressing cells in murine brain by immunofluorescence.
Image caption:
a Fluorescence micrographs of coronal brain sections from juvenile (P15) and early adult (P30) miR-145+/+ and miR-145–/– animals. Sections stained for MBP (green), counterstained with Dapi (white). Scale bar = 500μm. c Quantifications of the numbers of myelinated axons per 100 μm2 in corpus callosum from P15, P30 and P60 miR-145+/+ and miR-145–/– animals.
From: Kornfeld SF, Cummings SE, Yaworski R, De Repentigny Y, Gagnon S, Zandee S, Fathi S, Prat A, Kothary R.
Loss of miR-145 promotes remyelination and functional recovery in a model of chronic central demyelination.
Commun Biol. 2024 Jul 4;7(1):813.
doi: 10.1038/s42003-024-06513-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti myelin basic protein antibody, clone 14 (MCA408S) used to identify myelin basic protein expression in murine oligodendrocytes by immunofluorescence.
Image caption:
Fluorescent micrographs of lentivirus transduced OLs on differentiation day 2.5 (DD2.5; a) and differentiation day 5 (DD5; b). Left panels—miR-ctl; right panels—miR-145. GFP (green) indicates transduced cells; additional staining for myelin basic protein (MBP; magenta), Olig2 (red), counterstained with Hoechst (blue). Scale bar = 100 μm. Confocal micrographs of single OLs on DD2.5 (g) stained for MBP. Left panels—miR-ctl; right panels—miR-145. Scale bars = 50 μm.
From: Kornfeld SF, Cummings SE, Yaworski R, De Repentigny Y, Gagnon S, Zandee S, Fathi S, Prat A, Kothary R.
Loss of miR-145 promotes remyelination and functional recovery in a model of chronic central demyelination.
Commun Biol. 2024 Jul 4;7(1):813.
doi: 10.1038/s42003-024-06513-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti MBP (aa36-50)
- Product Type
- Monoclonal Antibody
- Clone
- 14
- Isotype
- IgG2b
- Specificity
- MBP
- Region
- (aa36-50)
Antibody Quality and Performance Guarantee
| Rat anti MBP antibody, clone 14 recognizes myelin basic protein (MBP), a component of myelin that is beleived to play a role in the myelination of nerves in the central nervous system. Rat anti MBP antibody, clone 14 has also been reported to work in western blotting (Relucio et al. 2009). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Chicken Expected from Sequence Horse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- 0.1M TRIS
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Bovine myelin basic protein
- Fusion Partners
- Spleen cells from immunized outbred rats were fused with cells of the mouse NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for MBP antibody
-
Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
J Neuroimmunol. 12 (4): 253-64. -
Glynn, P. et al. (1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.
J Neurochem. 48 (3): 752-9. -
Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
J Neuroimmunol. 19 (4): 305-15. -
Matsuo, A. et al. (1997) Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.
Am J Pathol. 150: 1253-66. -
Jackson, S.J. et al. (2004) Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture.
Eur J Neurosci. 20: 2267-75. -
Friess, M. et al. (2016) Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.
Cell Calcium. 60 (5): 322-30. -
Ou-yang, M.H. et al. (2015) N-terminal region of myelin basic protein reduces fibrillar amyloid-β deposition in Tg-5xFAD mice.
Neurobiol Aging. 36 (2): 801-11. -
Biffi, A. et al. (2006) Gene therapy of metachromatic leukodystrophy reverses neurological damage and deficits in mice.
J Clin Invest. 116 (11): 3070-82.
View The Latest Product References
-
Copray, J.C. et al. (2005) p75NTR independent oligodendrocyte death in cuprizone-induced demyelination in C57BL/6 mice.
Neuropathol Appl Neurobiol. 31 (6): 600-9. -
Jagielska, A. et al. (2017) Mechanical Strain Promotes Oligodendrocyte Differentiation by Global Changes of Gene Expression.
Front Cell Neurosci. 11: 93. -
Dias, D.O. et al. (2021) Pericyte-derived fibrotic scarring is conserved across diverse central nervous system lesions.
Nat Commun. 12 (1): 5501. -
Kornfeld, S.F. et al. (2024) Loss of miR-145 promotes remyelination and functional recovery in a model of chronic central demyelination.
Commun Biol. 7 (1): 813.
- Synonyms
- Myelin Basic Protein
- RRID
- AB_325007
- UniProt
- P02687
- Entrez Gene
- MBP
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0019911 structural constituent of myelin sheath
- GO:0043209 myelin sheath
MCA408S
147864
158903
166210
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