MBP antibody | 12

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Rat anti MBP (aa82-87)

Product Type
Monoclonal Antibody
Clone
12
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA409S E IF R WB 2 ml
Rat anti MBP antibody, clone 12 recognizes myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).

Rat anti MBP antibody, clone 12 has been reported as being suitable for use in Western blotting (Glynn et al. 1987).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
Mammals
Rat
Guinea Pig
Sheep
Human
Chicken
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
0.1M TRIS
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Bovine MBP.
Fusion Partners
Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02687 Related reagents
Entrez Gene
MBP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath
Regulatory
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Radioimmunoassays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for MBP antibody

  1. Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
    J Neuroimmunol. 12 (4): 253-64.
  2. Glynn, P. et al. (1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.
    J Neurochem. 48 (3): 752-9.
  3. Hruby, S. et al. (1987) Monoclonal antibodies reactive with myelin basic protein.
    Mol Immunol. 24 (12): 1359-64.
  4. Homchaudhuri L et al. (2009) Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.
    Biochemistry. 48 (11): 2385-93.
  5. Pohl, H.B. et al. (2011) Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.
    J Neurosci. 31 (3): 1069-80.
  6. Laursen, L.S. et al. (2011) Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP-K.
    J Cell Biol. 192: 797-811.
  7. Relucio, J. et al. (2009) Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development.
    J Neurosci. 29: 11794-806.
  8. Savvaki, M. et al. (2010) The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.
    J Neurosci. 30: 13943-54.
  9. Massa, P.T. et al. (2004) Dysmyelination and reduced myelin basic protein gene expression by oligodendrocytes of SHP-1-deficient mice.
    J Neurosci Res. 77: 15-25.
  10. Monk, K.R. et al. (2011) Gpr126 is essential for peripheral nerve development and myelination in mammals.
    Development. 138: 2673-80.
  11. Massa, P.T. et al. (2002) Critical role for protein tyrosine phosphatase SHP-1 in controlling infection of central nervous system glia and demyelination by Theiler's murine encephalomyelitis virus.
    J Virol. 76:8335-46.
  12. Relvas, J.B. et al. (2001) Expression of dominant-negative and chimeric subunits reveals an essential role for beta1 integrin during myelination.
    Curr Biol. 11: 1039-43.
  13. Rittchen S et al. (2015) Myelin repair in vivo is increased by targeting oligodendrocyte precursor cells with nanoparticles encapsulating leukaemia inhibitory factor (LIF).
    Biomaterials. 56: 78-85.
  14. Ioannidou, K. et al. (2012) Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.
    PLoS One. 7: e30775.
  15. Horn, M. et al. (2012) Myelin is dependent on the Charcot-Marie-Tooth Type 4H disease culprit protein FRABIN/FGD4 in Schwann cells.
    Brain. 135 (Pt 12): 3567-83.
  16. Müller, C. et al. (2015) SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.
    PLoS One. 10 (8): e0136900.
  17. Brügger V et al. (2015) HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
    PLoS Biol. 13 (9): e1002258.
  18. Natrajan, M.S. et al. (2015) Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
    Brain. 138(Pt 12):3581-97
  19. Fernandes, A.R. & Chari, D.M. (2016) Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy.
    J Control Release. 238: 289-99.
  20. Friess, M. et al. (2016) Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.
    Cell Calcium. Jun 29. pii: S0143-4160(16)30100-2. [Epub ahead of print]
  21. Fernandes, A.R. & Chari, D.M. (2016) Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology.
    J Control Release. 238: 300-10.
  22. Crawford, A.H. et al. (2016) Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.
    Am J Pathol. 186 (3): 511-6.
  23. Lim, J.L. et al. (2016) Protandim Protects Oligodendrocytes against an Oxidative Insult.
    Antioxidants (Basel). 5 (3): pii: E30.
  24. Isoda, M. et al. (2016) Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.
    Neurosci Res. 110: 18-28.
  25. Grigoletto, J. et al. (2017) Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss.
    Acta Neuropathol Commun. 5 (1): 37.
  26. Moreno, B. et al. (2017) Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
    Multiple Sclerosis Demyelinating Disorders. 2 (1): .
  27. Qin, J. et al. (2017) GD1a Overcomes Inhibition of Myelination by Fibronectin via Activation of Protein Kinase A: Implications for Multiple Sclerosis.
    J Neurosci. 37 (41): 9925-38.
  28. Aranmolate, A. et al. (2017) Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy.
    BMC Neurosci. 18 (1): 63.
  29. Yu, Q. et al. (2017) Strain differences in cuprizone induced demyelination.
    Cell Biosci. 7: 59.
  30. Dillenburg, A. et al. (2018) Activin receptors regulate the oligodendrocyte lineage in health and disease.
    Acta Neuropathol. Feb 03 [Epub ahead of print].