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MBP antibody | 12

Rat anti MBP (aa82-87)

Product Type
Monoclonal Antibody

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SDS Safety Datasheet SDS
E IF R WB 2 ml loader
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Rat anti MBP antibody, clone 12 recognizes myelin basic protein from a wide range of species. Rat anti MBP antibody, clone 12 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well with Rat anti MBP antibody, clone 12, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).

Rat anti MBP antibody, clone 12 has been reported as being suitable for use in western blotting (Glynn et al. 1987).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Preservative Stabilisers
0.1%Sodium Azide
Bovine MBP.
Fusion Partners
Spleen cells from an immunized outbred rat were fused with cells of the mouse NS0 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for MBP antibody

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  9. View The Latest Product References
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Immunohistology - Paraffin

Western Blotting

Myelin Basic Protein
Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath


151723B 153560 154086 158445 158446 165075 165076

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