MBP antibody | 12

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Rat anti MBP (aa82-87)

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
2 ml loader

Rat anti MBP antibody, clone 12 recognizes myelin basic protein from a wide range of species. Rat anti MBP antibody, clone 12 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well with Rat anti MBP antibody, clone 12, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).

Rat anti MBP antibody, clone 12 has been reported as being suitable for use in western blotting (Glynn et al. 1987).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Preservative Stabilisers
0.1%Sodium Azide
Bovine MBP.
Fusion Partners
Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images


Immunohistology - Paraffin

Western Blotting

Product Specific References

References for MBP antibody

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    J Neuroimmunol. 12 (4): 253-64.
  2. Glynn, P. et al. (1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.
    J Neurochem. 48 (3): 752-9.
  3. Hruby, S. et al. (1987) Monoclonal antibodies reactive with myelin basic protein.
    Mol Immunol. 24 (12): 1359-64.
  4. Homchaudhuri L et al. (2009) Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.
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  5. Pohl, H.B. et al. (2011) Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.
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  6. Laursen, L.S. et al. (2011) Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP-K.
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  27. Qin, J. et al. (2017) GD1a Overcomes Inhibition of Myelination by Fibronectin via Activation of Protein Kinase A: Implications for Multiple Sclerosis.
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  28. Aranmolate, A. et al. (2017) Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy.
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  29. Yu, Q. et al. (2017) Strain differences in cuprizone induced demyelination.
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  31. Sekizar, S. & Williams, A. (2019) Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord.
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  33. Nocita, E. et al. (2019) EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.
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    Glia. 68 (5): 932-46.
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  36. Swire, M. et al. (2019) Endothelin signalling mediates experience-dependent myelination in the CNS.
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  37. di Petna, A. et al. (2013) Oxidative stress and proinflammatory cytokines contribute to demyelination and axonal damage in a cerebellar culture model of neuroinflammation.
    PLoS One. 8 (2): e54722.
  38. Melero‐Jerez, C. et al. (2020) Myeloid‐derived suppressor cells support remyelination in a murine model of multiple sclerosis by promoting oligodendrocyte precursor cell survival, proliferation, and differentiation.
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  39. Klein, B. et al. (2020) DCX+ neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus.
    Sci Rep. 10 (1): 20095.
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  41. Werkman, I.L. et al. (2020) Transcriptional heterogeneity between primary adult grey and white matter astrocytes underlie differences in modulation of in vitro myelination.
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  42. Kerman, B.E. et al. (2020) Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination.
    J Biol Chem. 295 (34): 12233-46.
  43. Holloway, R.K. et al. (2021) Microglial inflammasome activation drives developmental white matter injury.
    Glia. Jan 08 [Epub ahead of print].
  44. Schultz, V. et al. (2021) Zika Virus Infection Leads to Demyelination and Axonal Injury in Mature CNS Cultures.
    Viruses. 13 (1): 91.
  45. Meireles, A.M. et al. (2018) The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex.
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  46. Lloyd, A.F. et al. (2019) Central nervous system regeneration is driven by microglia necroptosis and repopulation.
    Nat Neurosci. 22 (7): 1046-52.
  47. Yamazaki, R. et al. (2021) Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye.
    Sci Rep. 11 (1): 16906.
  48. Alhajlah, S. et al. (2021) Overexpression of Reticulon 3 Enhances CNS Axon Regeneration and Functional Recovery after Traumatic Injury.
    Cells. 10 (8): 2015.
  49. Swire, M. et al. (2021) Oligodendrocyte HCN2 Channels Regulate Myelin Sheath Length.
    J Neurosci. 41 (38): 7954-64.
  50. Niu, J. et al. (2021) Oligodendroglial ring finger protein Rnf43 is an essential injury-specific regulator of oligodendrocyte maturation.
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  51. Moyon, S. et al. (2021) TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice.
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  52. Bechler, M.E. (2019) A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.
    Methods Mol Biol. 1936: 97-110.
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    Methods Mol Biol. 1936: 169-83.
  54. Li, S. et al. (2019) Induction of immunological tolerance to myelinogenic glial-restricted progenitor allografts.
    Brain. 142 (11): 3456-72.
  55. Berghoff, S.A. et al. (2021) Microglia facilitate repair of demyelinated lesions via post-squalene sterol synthesis.
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  56. Kalafatakis, I. et al. (2021) The beneficial role of the synthetic microneurotrophin BNN20 in a focal demyelination model.
    J Neurosci Res. 99 (5): 1474-95.
  57. Grigoletto, J. et al. (2022) Loss of Corticostriatal Mu-Opioid Receptors in α-Synuclein Transgenic Mouse Brains
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