CD3 antibody | MM1A
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD3 antibody, clone MM1A (MCA6080) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653F).
Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bison Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Monocyte enriched bovine mononuclear cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q28073
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- Regulatory
- For research purposes only
Applications of CD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Immunocytochemistry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG1:RPE | STAR132PE | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD4 | MCA6081 | F | 0.1 mg |
|
Product Specific References
Source Reference
-
Davis, W.C. et al. (1993) Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
Vet Immunol Immunopathol. 39 (1-3): 85-91.
Antibody Characterization Reference
-
MacHugh N.D. et al. (1998) Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
Vet Immunol Immunopathol. 61 (1): 25-35.
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