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CD3 antibody | MM1A

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Anti Bovine CD3 Antibody, clone MM1A thumbnail image 1
Anti Bovine CD3 Antibody, clone MM1A gallery image 1
Flow cytometric staining of bovine peripheral blood lymphocytes.
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD3 antibody, clone MM1A (MCA6080) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653F).

Mouse anti Bovine CD3

Product Type
Monoclonal Antibody
Clone
MM1A
Isotype
IgG1
Specificity
CD3

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Product Code Applications Pack Size List Price Your Price Qty
MCA6080
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IC 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bison
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Monocyte enriched bovine mononuclear cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunocytochemistry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG1:RPE STAR132PE F 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG1:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine CD4 MCA6081 F 0.1 mg
List Price Your Price
Description Mouse anti Bovine CD4

Source Reference

  1. Davis, W.C. et al. (1993) Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
    Vet Immunol Immunopathol. 39 (1-3): 85-91.

Antibody Characterization Reference

  1. MacHugh N.D. et al. (1998) Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
    Vet Immunol Immunopathol. 61 (1): 25-35.

References for CD3 antibody

  1. Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle.
    Vet Immunol Immunopathol. 232: 110167.

UniProt
Q28073
Entrez Gene
CD3E
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway

MCA6080

153381 156576 160716 165371

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