CD3 antibody | MM1A
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD3 antibody, clone MM1A (MCA6080) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653F).
Filter by Application:
F ResetMouse anti Bovine CD3
- Product Type
- Monoclonal Antibody
- Clone
- MM1A
- Isotype
- IgG1
- Specificity
- CD3
Antibody Quality and 
Unrivaled 
| Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bison Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Monocyte enriched bovine mononuclear cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
| Immunocytochemistry | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG1:RPE | STAR132PE | F | 0.1 mg |
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| Description | Goat anti Mouse IgG1:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine CD4 | MCA6081 | F | 0.1 mg |
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| Description | Mouse anti Bovine CD4 | ||||||
Source Reference
-
Davis, W.C. et al. (1993) Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
Vet Immunol Immunopathol. 39 (1-3): 85-91.
Antibody Characterization Reference
-
MacHugh N.D. et al. (1998) Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
Vet Immunol Immunopathol. 61 (1): 25-35. Vet Immunol Immunopathol. 61 (1): 25-35.
References for CD3 antibody
-
Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle.
Vet Immunol Immunopathol. 232: 110167.
- UniProt
- Q28073
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
View more products with CD3 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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