CD3 antibody | MM1A

100% Secure

Anti Bovine CD3 Antibody, clone MM1A gallery image 1 — **Flow cytometric staining of bovine peripheral blood lymphocytes.**
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD3 antibody, clone MM1A (**MCA6080**) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653F).

Mouse anti Bovine CD3

Product Type: Monoclonal Antibody
Clone: MM1A
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA6080	F IC			0.1 mg

Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Bison
Water Buffalo

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Concentrated tissue culture supernatant - liquid

Preparation

Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter

Buffer Solution

Serum free tissue culture medium containing proprietary protein free supplement

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Monocyte enriched bovine mononuclear cells

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q28073 Related reagents
Entrez Gene: CD3E Related reagents
GO Terms: GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0007166 cell surface receptor linked signaling pathway
Regulatory: For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10
Immunocytochemistry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG1:RPE	STAR132PE	0.1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse anti Bovine CD4	MCA6081	0.1 mg	F

Product Specific References

Source Reference

Davis, W.C. et al. (1993) Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
Vet Immunol Immunopathol. 39 (1-3): 85-91.

Antibody Characterization Reference

MacHugh N.D. et al. (1998) Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
Vet Immunol Immunopathol. 61 (1): 25-35.

View more products with CD3 specificity