Bevacizumab antibody | AbD16748_hIgG1
Human anti Bevacizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD16748_hIgG1
- Isotype
- IgG1
- Specificity
- Bevacizumab
Human Anti-Bevacizumab Antibody, clone AbD16748_IgG1 is a paratope-specific, recombinant anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug bevacizumab and inhibits the binding of the drug to its target, vascular endothelial growth factor A (VEGF-A). The antibody can be used to measure the levels of bevacizumab and biosimilars in serum from patients. A pair of anti-bevacizumab antibodies can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free drug. This antibody is recommended as the detection antibody, paired with Anti-Bevacizumab Antibody in monovalent Fab format, clone AbD17976 (HCA182) as the capture antibody. Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian. View a summary of all Anti-Bevacizumab Antibodies. |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- HCA184G: Purified IgG prepared by affinity chromatography on Protein A
- HCA184: Purified IgG prepared by affinity chromatography on Protein A.
- Source
- HKB-11
- Buffer Solution
- HCA184G: Phosphate buffered saline
- HCA184: Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Bevacizumab
- Purity
- HCA184G: 97.8% (capillary electrophoresis, reducing conditions)
- HCA184: 97.8% (capillary electrophoresis, reducing conditions).
- Affinity
- HCA184G: The intrinsic affinity of the monovalent form of this antibody is KD=2.1nM as measured by real time, label-free molecular interaction analysis on immobilized Bevacizumab.
- HCA184: The monovalent intrinsic affinity of this antibody is KD=2.1nM as measured by real time, label-free molecular interaction analysis on immobilized Bevacizumab.
- Approx. Protein Concentrations
- HCA184G: Antibody concentration 1.0 mg/ml
- HCA184: Antibody concentration 0.5 mg/ml.
- Regulatory
- HCA184G: For research purposes only
- HCA184: For research purposes only.
- Guarantee
- HCA184G: 12 months from date of despatch
- HCA184: 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Avastin is a trademark of Genentech, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA182 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human anti Bevacizumab | HCA182 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Bevacizumab | ||||||
Human anti Bevacizumab | HCA185 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Bevacizumab | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | LYNX Rapid HRP Antibody Conjugation Kit |
References for Bevacizumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1 -
Lallemand, C. et al. (2020) Quantification of Bevacizumab Activity Following Treatment of Patients With Ovarian Cancer or Glioblastoma.
Front Immunol. 11: 515556.
- Synonyms
- Avastin
HCA184G
148601 1709HCA184
155646 1609If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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