Method to determine LD50 using alamarBlue®
alamarBlue® can be used to determine the median lethal dose LD50 value and similar assays testing the effects of a toxin, radiation, or pathogen against cell cultures.
Percentage difference in reduction (equation 5)
ALW - (AHW x RO) for test well
ALW - (AHW x RO) for control well
Use semi-log graph paper to plot the percent of untreated control (using equation 5) for each dilution of a given test agent on the y-axis vs. the concentration of the test agent on the x-axis.
To determine the LD50 endpoint from the graph, read from where the 50 percent point intercepts the Dose.
Response Curve to the concentration along the x-axis. This concentration is the LD50 value.
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