Somatostatin Antibody | YC7

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Somatostatin Antibody | YC7 gallery image 1

Published customer image:
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b) and juvenile zebrafish gut (c, d) using antibody against galanin (a–d) and neuronal marker Hu (a, d), somatostatin (Sst) (c). Red fluorescence protein (RFP) marked mnx1+ population of β cells (b, b'). Arrows show galaninergic neurons in the autonomic ganglion. In the primary islet of the endocrine pancreas, galanin-IR fibers formed a very dense network (arrowheads). CrG cranial ganglia, E eye, I intestine, Me mesencephalon, Rho rhombencephalon, Y yolk. Scale bars 100 μm

From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.

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Somatostatin Antibody | YC7 gallery image 2

Published customer image:
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b) and juvenile zebrafish gut (c, d) using antibody against galanin (a–d) and neuronal marker Hu (a, d), somatostatin (Sst) (c). Red fluorescence protein (RFP) marked mnx1+ population of β cells (b, b'). Arrows show galaninergic neurons in the autonomic ganglion. In the primary islet of the endocrine pancreas, galanin-IR fibers formed a very dense network (arrowheads). CrG cranial ganglia, E eye, I intestine, Me mesencephalon, Rho rhombencephalon, Y yolk. Scale bars 100 μm

From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.

Enlarge
Somatostatin Antibody | YC7 gallery image 3

Published customer image:
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish larvae whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b, d), juvenile (c, e), and adult (f) guts using antibody against galanin (a–c, e–f), cholinergic marker—choline acetyltransferase (ChAT) (a), δ cell marker somatostatin (b–d). RFP marked mnx1+ population of β cells in the transgenic line of the zebrafish (d, e). Arrows point to galaninergic neurons in autonomic ganglia. Arrowheads show the primary islet of the endocrine pancreas, and hollow arrows point to the secondary islets. a There was no ChAT immunoreactivity in the cells and fibers associated with the pancreas and intestine. ChAT-IR neurons were observed only in the brain and spinal cord (not shown). Galanin-IR fibers were also visible, projecting from the autonomic ganglion to the intestine. c, d Pancreatic islets at a higher magnification. Somatostatin did not colocalize with β cell marker (d). Delta cells were very intensely supplied by galaninergic fibers (c, d). Note that the secondary islets did not contain RFP+ cells until 60 dpf (e). Dashed line indicates the pancreas. I intestine, L liver, Rho rhombencephalon. Scale bars 100 μm, except d = 50 μm

From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.

Enlarge
Somatostatin Antibody | YC7 gallery image 4

Published customer image:
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish tissues by immunofluorescence.
Image caption:
Section from the adult pancreas stained with antibody against galanin (a–c) and Hu (b), and somatostatin (c). RFP marked mnx1+ population of β cells in the transgenic line of the zebrafish (a–c). RFP+ cells were present mostly only in one (primary) islet, whereas somatostatin-positive cells were located in all the islets (a–c). Ganglion containing galanin-IR neurons is visible outside the pancreatic tissue, close to the intestinal wall (a, a?', c, c'). Neurons inside the pancreatic tissue are also visible; however, they were mostly galanin-negative (arrowheads, b). Galanin-IR fibers richly supplied pancreatic islets (c). I intestine, Pi pancreatic islet. Scale bars 100 μm

From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.

Enlarge
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  • Rat anti Human Somatostatin
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  • Product Type
    Monoclonal Antibody
  • Clone
    YC7
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8330-0009C, Pdatasheet pdfdatasheet pdf0.2 ml
    8330-0009
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Human Somatostatin antibody, clone YC7 is specific for somatostatin. A hormone involved in endocrine system regulation through interactions with pituitary growth hormone, thyroid stimulating hormone, and a number of hormones of the gastrointestinal tract. Somatostatin has active 14 amino acid and 28 amino acid forms, produced by alternate cleavage of the gene product.

      This antibody does not cross react with enkephalins, other endorphins, substance P, or CGRP. It partially cross reacts with somatostatin fragments.

      The epitope recognised by this antibody lies within the 1-14 amino acid sequence of cyclic somatostatin.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Guinea Pigyes
      Pigyes
      Mouseyes
      Zebrafishyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05% Thiomersal
    • Immunogen
      Somatostatin conjugated to thyroglobulin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from rats immunised with somatostatin conjugated to thyroglobulin were fused with a rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      response to nutrient
      negative regulation of cell proliferation
      G-protein coupled receptor protein signaling pathway
      extracellular space
      digestion
      induction of apoptosis by hormones
      hormone activity
      synaptic transmission
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Somatostatin Antibody Formats

    Formats Clone Applications Sizes available
    Somatostatin Antibody : S/N YC7 C, P 0.2 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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