Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 (MCA1662) as a capture reagent and biotinylated Goat anti Human IgM AHP1764) as a detection regent with purified human IgM (5275-5504) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown
Goat anti Human IgM antibody recognizes human IgM and shows minimal cross-reactivity with other human immunoglobulin classes. This antiserum has not been cross adsorbed and may react with the mu chain of IgM from other species. The Ig mu chain is a 452 amino acid ~50kDa member of the immunoglobulin superfamily expressed as a single pass type I transmembrane molecule on the surface of B-cells and as a circulating secreted form (UniProt: P01871)
Goat anti Human IgM antibody has been successfully used as a capture reagent in the development of a sensitive sandwich ELISA for the evaluation of IgM levels in human samples.
Goat anti Human IgM antibody is available in bulk quantities and may be made available in other formats. Please enquire for further details.
Serum - liquid
Antisera to Human IgM were raised by repeated immunisation of goats with highly purified antigen.
0.1% Sodium Azide (NaN3)
Purified human IgM.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.