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IgM Antibody

IgM Antibody gallery image 1

Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 (MCA1662) as a capture reagent and biotinylated Goat anti Human IgM AHP1764) as a detection regent with purified human IgM (5275-5504) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown

  • IgM Antibody thumbnail image 1
  • Goat anti Human IgM
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1764E, TBdatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human IgM antibody recognizes human IgM and shows minimal cross-reactivity with other human immunoglobulin classes. This antiserum has not been cross adsorbed and may react with the mu chain of IgM from other species. The Ig mu chain is a 452 amino acid ~50kDa member of the immunoglobulin superfamily expressed as a single pass type I transmembrane molecule on the surface of B-cells and as a circulating secreted form (UniProt: P01871)

      Goat anti Human IgM antibody has been successfully used as a capture reagent in the development of a sensitive sandwich ELISA for the evaluation of IgM levels in human samples.

      Goat anti Human IgM antibody is available in bulk quantities and may be made available in other formats. Please enquire for further details.
    • Intended Use
    • Target Species
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human IgM were raised by repeated immunisation of goats with highly purified antigen.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Purified human IgM.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      antigen binding
      membrane fraction
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgM Antibody Formats

    Formats Applications Sizes available
    IgM Antibody : Serum E, TB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IgM Antibody thumbnail image 1

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