IgM

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Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 (MCA1662) as a capture reagent and biotinylated Goat anti Human IgM (AHP1764) as a detection regent with purified human IgM (5275-5504) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown

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  • Native Human IgM
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  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5275-5504Edatasheet pdfdatasheet pdf5 mg
    5275-5504
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Native Human IgM is purified from human plasma and demonstrated a single band in electrophoresis. Native Human IgM has been used as the IgM antigen in the development of a sensitive ELISA assay for the measurement of IgM in human samples.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified immunoglobulin from human plasma - lyophilised
    • Reconstitution
      Reconstitute with 1ml 250mM Sodium Chloride. Further dilutions may then be made in sterile Phosphate Buffered Saline.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgM prepared by delipidation, salt fractionation and ion-exchange chromatography.
    • Preservative Stabilisers
      None present.
    • Purity
      >95% Single band in high resolution protein electrophoresis on Corning HRPE agarose gel. 0.5 µl of IgM solution at 50 mg/ml in isotonic saline.
    • Approx. Protein Concentrations
      5.0 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      membrane fraction
      antigen binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgM Formats

    Formats Applications Sizes available
    IgM : Purified E 5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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