100% secure
Email this product page
Print this page

G-CSF Antibody | BVD13-3A5

G-CSF Antibody | BVD13-3A5 gallery image 1

Sandwich ELISA analysis of Human G-CSF using Rat anti human G-CSF (1012701) as a capture reagent and biotinylated Rat anti Human G-CSF (1012808) as a detection reagent with recombinant human G-CSF (PHP082) as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human serum (orange) and plasma (green) samples are tested undiluted

  • G-CSF Antibody | BVD13-3A5 thumbnail image 1
  • Rat anti Human G-CSF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1012701E, WBdatasheet pdfdatasheet pdf0.5 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).

      G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.

      Rat anti Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-37G10 as a detection reagent and recombinant human G-CSF as antigen.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml5ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with 1012808 as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional G-CSF Antibody Formats

    Formats Clone Applications Sizes available
    G-CSF Antibody : Purified BVD13-3A5 E, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • G-CSF Antibody | BVD13-3A5 thumbnail image 1

              Write your review

              You may also be interested in...