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EMR1 antibody | A10

Product Code Applications Pack Size List Price Your Price Qty
MCA2674GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
MCA2674
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SDS Safety Datasheet SDS
F 0.2 mg loader
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loader

Hamster anti Human EMR1 antibody, clone A10 recognizes human EMR1 (EGF-like module containing, mucin-like, hormone receptor-like 1). EMR1 is a seven-span transmembrane protein which is a member of the EGF-TM7 family of receptors. EMR1 is the human ortholog of murine F4/80 but unlike F4/80, the expression of EMR1 in humans is restricted to eosinophils and is not expressed on macrophage populations. The detailed structure and function of EMR1 is still unknown.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Fibroblast cell line ARH102 expressing CD97-EMR1 (EGF1-6)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550
Goat F(ab')2 anti Hamster IgG:DyLight®650 STAR104D650 F IF 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®650
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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
>
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Description Hamster (Armenian) IgG Negative Control

Source Reference

  1. Hamann, J. et al. (2007) EMR1, the human homolog of F4/80, is an eosinophil-specific receptor.
    Eur J Immunol. 37 (10): 2797-802.

References for EMR1 antibody

  1. van Eijk, M. et al. (2010) Differential expression of the EGF-TM7 family members CD97 and EMR2 in lipid-laden macrophages in atherosclerosis, multiple sclerosis and Gaucher disease.
    Immunol Lett. 129 (2): 64-71.
  2. Pothoven, K.L. et al. (2017) Neutrophils are a major source of the epithelial barrier disrupting cytokine oncostatin M in patients with mucosal airways disease.
    J Allergy Clin Immunol. 139 (6): 1966-78.e9.
  3. Fuchs, A. et al. (2021) Associations Among Adipose Tissue Immunology, Inflammation, Exosomes and Insulin Sensitivity in People With Obesity and Nonalcoholic Fatty Liver Disease.
    Gastroenterology. 161 (3): 968-981.e12.

RRID
AB_10891443
UniProt
Q14246
Entrez Gene
EMR1
GO Terms
GO:0005509 calcium ion binding
GO:0007155 cell adhesion
GO:0004930 G-protein coupled receptor activity
GO:0005887 integral to plasma membrane
GO:0007218 neuropeptide signaling pathway

MCA2674GA

0807R 155269

MCA2674

150103 154519

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