CD235a Antibody | YTH89.1

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CD235a Antibody | YTH89.1 gallery image 1

CD235a (Glycophorin A) - MCA506G - Human hypophysis showing erythrocyte labelling in sinusoids. Formalin fixed, paraffin processed tissue

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CD235a Antibody | YTH89.1 gallery image 2

Staining of human peripheral blood erythrocytes with Rat anti Human CD235a:FITC (MCA506F)

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CD235a Antibody | YTH89.1 gallery image 3

Staining of human peripheral blood erythrocytes with Rat anti Human CD235a:RPE (MCA506PE)

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CD235a Antibody | YTH89.1 gallery image 4

Staining of red blood cells cells with Rat anti Human CD235a:Alexa Fluor® 647 (MCA506A647)

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  • Rat anti Human CD235a:RPE
  • Rat anti Human CD235a:FITC
  • Rat anti Human CD235a:Alexa Fluor® 647
  • Rat anti Human CD235a:Alexa Fluor® 647
  • Rat anti Human CD235a
  • Rat anti Human CD235a:FITC
  • Rat anti Human CD235a:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YTH89.1
  • Isotype
    IgG2b
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA506FFdatasheet pdfdatasheet pdf0.1 mg
    MCA506F
    MCA506GC, CT, F, Pdatasheet pdfdatasheet pdf0.2 mg
    MCA506G
    MCA506PEFdatasheet pdfdatasheet pdf100 Tests
    MCA506PE
    MCA506A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA506A647
    MCA506FTFdatasheet pdfdatasheet pdf25 µg
    MCA506FT
    MCA506PETFdatasheet pdfdatasheet pdf25 Tests
    MCA506PET
    MCA506A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA506A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Human CD235a antibody, clone YTH89.1 recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      identical protein binding
      plasma membrane
      membrane fraction
      integral to membrane
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/101/25
      Immunohistology - Frozen1/1001/1000
      Immunohistology - Paraffin1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human hypophysis
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD235a Antibody Formats

    Formats Clone Applications Sizes available
    CD235a Antibody : Alexa Fluor® 647 YTH89.1 F 100 Tests/1ml | 25 Tests/0.25ml
    CD235a Antibody : RPE YTH89.1 F 25 Tests | 100 Tests
    CD235a Antibody : Purified YTH89.1 C, CT, F, P 0.2 mg
    CD235a Antibody : FITC YTH89.1 F 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
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      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
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      STAR21B
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      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:RPEMCA6006PE100 TestsF
        MCA6006PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:FITCMCA6006F0.1 mgF
        MCA6006F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:Alexa Fluor® 647MCA6006A647100 Tests/1mlF
        MCA6006A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:Alexa Fluor® 647MCA6006A647100 Tests/1mlF
        MCA6006A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative ControlMCA6006GA0.1 mgF
        MCA6006GA
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:FITCMCA6006F0.1 mgF
        MCA6006F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:RPEMCA6006PE100 TestsF
        MCA6006PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Human hypophysis
              • Application NameReference Images
                Flow Cytometry

              References

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