IgG Antibody

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IgG Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Horse IgGT antibody used for the determination of allergen specific antibody titre in horse serum by ELISA.
Image caption:
Healthy horses have allergen-specific serum antibodies. Allergen-specific antibody titers, for different isotypes, were determined in the serum of healthy (dark bars) and IBH-sensitive animals (dark bars) by ELISA. Bars represent average +SEM; n = 6 and n = 10, for healthy and IBH-sensitive animals, respectively; dots represent individual animals.

From: Meulenbroeks C, van der Lugt JJ, van der Meide NMA, Willemse T, Rutten VPMG, et al. (2015) Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity. PLoS ONE 10(4): e0122090.

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  • IgG Antibody thumbnail image 1
  • Goat anti Horse IgG (T):Alk. Phos.
  • Goat anti Horse IgG (T):HRP
  • Goat anti Horse IgG (T)
  • Goat anti Horse IgG (T):FITC
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI38ABEdatasheet pdfdatasheet pdf0.5 mg
    AAI38AB
    AAI38PEdatasheet pdfdatasheet pdf1 mg
    AAI38P
    AAI38E, IDdatasheet pdfdatasheet pdf1 mg
    AAI38
    AAI38FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI38F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Horse IgG (T) antibody recognizes equine IgG (T). No cross-reactivity with other equine immunoglobulin classes is seen in immuno-electrophoresis.

      Goat anti Horse IgG (T) antibody may cross react with IgG from other species.
    • Intended Use
    • Target Species
      Horse
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to equine IgG (T) were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
    • Immunogen
      Purified equine IgG (T).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/100
      Immunohistology - Frozen1/201/200

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP E 1 mg
    IgG Antibody : FITC C, F 1 mg
    IgG Antibody : Purified E, ID 1 mg
    IgG Antibody : Alk. Phos. E 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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