Golimumab Antibody | AbD25429

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Golimumab Antibody | AbD25429 gallery image 1

Human Anti-Golimumab Antibody specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Golimumab Antibody, clone AbD25429 at a concentration of 2 μg/ml followed by Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.

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Human Anti-Golimumab Antibody specificity titration ELISA

A microtiter plate was coated overnight with golimumab, human TNFα, human IgG1/kappa, human IgG1/lambda or adalimumab at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Golimumab Antibody, clone AbD25429 titrated to the given concentrations in PBST followed by Mouse Anti-Penta Histidine Tag:HRP (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.

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Human Anti-Golimumab Antibody bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25429 at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using Human Anti-Golimumab:HRP Antibody clone AbD25418_hIgG1 (HCA287P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.

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Human Anti-Golimumab inhibition ELISA

A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25429. After washing and blocking with PBST + 5% BSA, golimumab was added at the given concentrations, mixed either with 5-fold molar excess of TNα (black line) or without TNFα (red line). Golimumab still capable of binding to the Human Anti-Golimumab Antibody coated plate, was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of two measurements.

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  • Human anti Golimumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-Golimumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalysis of golimumab and biosimilars. It is suitable as a capture antibody in a PK bridging ELISA to detect free drug, paired with HCA287.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD25429
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA286Edatasheet pdfdatasheet pdf0.1 mg
      HCA286
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human Anti-Golimumab Antibody, clone AbD25429 is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug golimumab and inhibits it binding to its target, TNFα. This antibody can be used to measure the levels of free golimumab and biosimilar products in bioanalytical assays.

        Clone AbD25429 is an affinity-matured variant of clone AbD20710 (HCA240), with about 2-fold affinity improvement.

        A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with the antibody in full immunoglobulin format, clone AbD25418_hIgG1 (HCA287P), as the detection antibody.

        Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

        View a summary of all anti-golimumab antibodies
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Golimumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of AbD25429 was measured as KD = 1.0 nM by real time, label free molecular interaction analysis on immobilized golimumab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        Clone AbD25429 can be used in a direct or indirect ELISA system or as capture antibody for golimumab in a bridging ELISA together with HCA287P (AbD25418_hIgG1) as the detection reagent.

        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Golimumab Antibody Formats

      Formats Clone Applications Sizes available
      Golimumab Antibody : Purified AbD25429 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Golimumab:HRPHCA287P0.1 mgE
            HCA287P
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
            MCA5995P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti GolimumabHCA2870.1 mgE
            HCA287

            Recommended Positive Controls

              Histology Controls

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