CD4 antibody | RPA-T4
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Mouse anti Human CD4:RPE-Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
|Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.
Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
- Reconstitute with 1 ml distilled water
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Human PHA blasts
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE-Alexa Fluor®647 488nm laser 496 667 RPE-Alexa Fluor®647 561nm laser 546 667
- For research purposes only
- 12 months from date of despatch
- The Alexa Fluor dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Tandem conjugates of RPE-Alexa Fluor 647 display fluorescence properties that are virtually identical to those of RPE-Cy5 tandem conjugates.
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- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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- Select combined or multi-laser view to visualize the spectra
References for CD4 antibody
Piatier-Tonneau, D. (1997) CD4 workshop panel report.
In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.
Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J Immunol. 167 (8): 4593-9.
Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
Immunology. 102 (2): 173-9.
Pentón-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
Int Immunopharmacol. 11 (1): 29-38.
Zhang, Y. et al. (2013) Accelerated in vivo. proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
PLoS Pathog. 9 (4): e1003310.
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088.
Agrawal, S.M. et al. (2013) Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.
Brain. 136 (Pt 6): 1760-77.
Malmassari, S.L. et al. (2007) Impact of hepatitis B virus basic core promoter mutations on T cell response to an immunodominant HBx-derived epitope.
Hepatology. 45 (5): 1199-209.
View The Latest Product References
Wooldridge, L. et al. (2006) Anti-coreceptor antibodies profoundly affect staining with peptide-MHC class I and class II tetramers.
Eur J Immunol. 36 (7): 1847-55.
Wildum, S. et al. (2006) Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection.
J Virol. 80 (16): 8047-59.
Kirchhof, J. et al. (2018) Learned immunosuppressive placebo responses in renal transplant patients.
Proc Natl Acad Sci U S A. 115 (16): 4223-7.
Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
J Cancer Therapy. 02 (01): 54-64.
- Entrez Gene
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005201 extracellular matrix structural constituent
- GO:0005769 early endosome
- GO:0005789 endoplasmic reticulum membrane
- GO:0006948 induction by virus of host cell-cell fusion
- View More GO Terms
- GO:0006955 immune response
- GO:0008270 zinc ion binding
- GO:0015026 coreceptor activity
- GO:0019059 initiation of viral infection
- GO:0019901 protein kinase binding
- GO:0030260 entry into host cell
- GO:0031295 T cell costimulation
- GO:0032507 maintenance of protein location in cell
- GO:0042101 T cell receptor complex
- GO:0042289 MHC class II protein binding
- GO:0042803 protein homodimerization activity
- GO:0045058 T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045234 protein palmitoleylation
- GO:0045860 positive regulation of protein kinase activity
- GO:0050690 regulation of defense response to virus by virus
- GO:0050852 T cell receptor signaling pathway
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