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CD45 Antibody | YKIX716.13
Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:FITC (MCA1042F)
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Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:Alexa Fluor®488 (MCA1042A488)
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Published customer image:
Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?±?0.21% (n?=?3). Average CD45+: 0.19?±?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).
From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.
Published customer image:
Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?±?0.21% (n?=?3). Average CD45+: 0.19?±?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).
From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.
Figure A. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11b (MCA1777S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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Figure A. PE conjugated Mouse anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on monocytes.
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Product TypeMonoclonal Antibody
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CloneYKIX716.13
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IsotypeIgG2b
7 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Rat anti Dog CD45 antibody, clone YKIX716.13 recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of 180/200kD from Con-A blasts (Cobbold et al. 1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform.
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Intended Use
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Target SpeciesDog
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Product FormPurified IgG conjugated to Alexa Fluor® 488 - liquidPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquidPurified IgG conjugated to Alexa Fluor® 647 - liquidPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilizedPurified IgG conjugated to Allophycocyanin (APC) - lyophilisedPurified IgG conjugated to Biotin - liquidPurified IgG - liquid
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ReconstitutionReconstitute with 1ml distilled waterReconstitute with 1ml distilled water
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PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin0.09% Sodium Azide 1% Bovine Serum Albumin 0.09% Sodium Azide 1% Bovine Serum Albumin 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose 0.09% Sodium Azide 1% Bovine Serum Albumin 0.09% Sodium Azide (NaN3) -
ImmunogenCanine thymocytes.
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Purity
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Approx. Protein ConcentrationsIgG concentration 0.05 mg/mlIgG concentration 0.1 mg/mlIgG concentration 0.05 mg/mlIgG concentration 0.1 mg/mlIgG concentration 1.0 mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered salinePhosphate buffered salinePhosphate buffered salinePhosphate buffered salinePhosphate buffered salinePhosphate buffered saline
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Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
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StorageStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.18 months from date of despatch.12 months from date of reconstitution.12 months from date of reconstitution.18 months from date of despatch.18 months from date of despatch.
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AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.comThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/5 Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/5 Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/5 Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/10 Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat Application Name Yes No Min Dilution Max Dilution Flow Cytometry 1/50 1/100 Immunoprecipitation - Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Technical Advice
- Technical Advice
- Technical Advice
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- ELISA
- ELISA
- ELISA
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Immunohistology
- Immunohistology
- Immunohistology
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Western Blotting
- Western Blotting
- Western Blotting
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
- Instructions For Use
- Instructions For Use
- Instructions For Use
- Instructions For Use
- Instructions For Use
Additional CD45 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD45 Antibody : RPE | YKIX716.13 | F | 100 Tests |
| CD45 Antibody : Biotin | YKIX716.13 | F | 0.1 mg |
| CD45 Antibody : APC | YKIX716.13 | F | 100 Tests |
| CD45 Antibody : Alexa Fluor® 647 | YKIX716.13 | F | 100 Tests/1ml |
| CD45 Antibody : FITC | YKIX716.13 | F | 0.1 mg |
| CD45 Antibody : Alexa Fluor® 488 | YKIX716.13 | F | 100 Tests/1ml |
| CD45 Antibody : Purified | YKIX716.13 | F, IP | 0.1 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Streptavidin:APC | STAR119 | 100 Tests | F | STAR119 |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C, E, P, WB | STAR131A | |
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C, E, IF, P, WB | STAR131B | |
| Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) | STAR71D549GA | 0.1 mg | F, IF | STAR71D549GA | |
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F, IF | STAR71D649GA | |
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F, IF, WB | STAR16D800GA | |
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F, IF, WB | STAR71D800GA | |
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | STAR69 | |
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | STAR17B | |
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C, E, P | STAR72 | |
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C, E, P, RE | STAR21B | |
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F | STAR73 | |
| Rabbit F(ab')2 anti Rat IgG:RPE | STAR20A | 1 ml | F | STAR20A |
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Rat IgG2b Negative Control:FITC | MCA6006F | 0.1 mg | F | MCA6006F |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Rat IgG2b Negative Control:Alexa Fluor® 647 | MCA6006A647 | 100 Tests/1ml | F | MCA6006A647 |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Rat IgG2b Negative Control:RPE | MCA6006PE | 100 Tests | F | MCA6006PE |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2b Negative Control:APC | MCA6006APC | 100 Tests | F | MCA6006APC |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2b Negative Control:Biotin | MCA6006B | 0.1 mg | F | MCA6006B |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | 0.1 mg | F | MCA6006GA |
Useful Reagents
Recommended Positive Controls
Histology Controls
References
1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.
2. Reis, A.B. et al (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clin Exp Immunol.146: 303-11.
3. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.Vet Microbiol. 131:237-46.
4. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.Am J Trop Med Hyg. 70: 618-24.
5. Modiano,J.F. and Helfand,S.C. (2011) Early detection of hemangiosarcoma and angiosarcomaPatent Application No.11/662529
6. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.J Vet Diagn Invest. 22: 438-41.
7. Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.J Nutr. 132: 1652S-4S.
8. Hunter, M.J. et al. (2011) Gene therapy of canine leukocyte adhesion deficiency using lentiviral vectors with human CD11b and CD18 promoters driving canine CD18 expression.Mol Ther. 19: 113-21.
9. Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.Vet Clin Pathol. 35: 47-54.
10. Giantin, M. et al. (2013) Evaluation of tyrosine-kinase receptor c-KIT (c-KIT) mutations, mRNA and protein expression in canine leukemia: might c-KIT represent a therapeutic target?Vet Immunol Immunopathol. 152: 325-32.
11. Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.BMC Vet Res. 9: 144.
12. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.Vet Comp Oncol. 12: 29-36.
13. Salinas Tejedor, L. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.Brain Behav Immun. pii: S0889-1591(15)00233-0.
14. Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.PLoS One. 11 (8): e0159095.
15. Poggi, A. et al. (2016) Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.Vet Comp Oncol. Jan 21. [Epub ahead of print]
16. Zeira, O. et al. (2015) Adult autologous mesenchymal stem cells for the treatment of suspected non-infectious inflammatory diseases of the canine central nervous system: safety, feasibility and preliminary clinical findings.J Neuroinflammation. 12: 181.
17. Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.Vet Immunol Immunopathol. 159 (1-2): 91-6.
18. Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.Vet Immunol Immunopathol. 155 (3): 211-7.
19. Nishimura, T. et al. (2017) Feeder-independent canine induced pluripotent stem cells maintained under serum-free conditions.Mol Reprod Dev. Feb 27. [Epub ahead of print]
20. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.Immunobiology. 221 (1): 12-22.
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