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MCP-1 Antibody

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Bovine MCP-1 ELISA

Recombinant Bovine MCP-1 (PBP024) detected using Rabbit anti Bovine MCP-1 (AHP2366) as the capture reagent and Goat anti Bovine MCP-1:Biotin (AHP2367B) as the detection reagent followed by Streptavidin:HRP

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  • Rabbit anti Bovine MCP-1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2366E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Bovine MCP-1 antibody recognizes bovine monocyte chemotactic protein-1 (MCP-1), also known as C-C motif chemokine 2 (CCL2). Bovine MCP-1 is a 99 amino acid, 11.1 kDa secreted protein.

      The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.

      MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine MCP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant bovine MCP-1
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.8 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      extracellular space
      chemokine activity
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5 ug/ml10 ug/ml
      Western Blotting0.1 ug/ml1.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2367B as the detection reagent. Recombinant bovine MCP-1 (PBP024) may be used as a standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MCP-1 Antibody Formats

    Formats Applications Sizes available
    MCP-1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Bovine MCP-1:BiotinAHP2367B50 µgE, WB
          Recombinant Bovine MCP-1PBP0245 µgE

          Recommended Positive Controls

            Histology Controls

                • Product Images

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