MCP-1 Antibody

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Bovine MCP-1 ELISA

Recombinant Bovine MCP-1 (PBP024) detected using Rabbit anti Bovine MCP-1 (AHP2366) as the capture reagent and Goat anti Bovine MCP-1:Biotin (AHP2367B) as the detection reagent followed by Streptavidin:HRP

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  • Goat anti Bovine MCP-1:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2367BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP2367B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Bovine MCP-1 antibody recognizes bovine monocyte chemotactic protein-1 (MCP-1), also known as C-C motif chemokine 2 (CCL2). Bovine MCP-1 is a 99 amino acid, 11.1 kDa secreted protein.

      The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.

      MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine MCP-1 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Recombinant bovine MCP-1
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      immune response
      extracellular space
      chemokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.1 ug/ml1.5 ug/ml
      Western Blotting1.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2366 as the capture reagent. Recombinant bovine MCP-1 (PBP024) may be used as a standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MCP-1 Antibody Formats

    Formats Applications Sizes available
    MCP-1 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Bovine MCP-1AHP23660.1 mgE, WB
          AHP2366
          Recombinant Bovine MCP-1PBP0245 µgE
          PBP024

          Recommended Positive Controls

            Histology Controls

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