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MCP-1 antibody

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Anti Bovine MCP-1 Antibody - Biotin gallery image 1
Bovine MCP-1 ELISA

Recombinant Bovine MCP-1 (PBP024) detected using Rabbit anti Bovine MCP-1 (AHP2366) as the capture reagent and Goat anti Bovine MCP-1:Biotin (AHP2367B) as the detection reagent followed by Streptavidin:HRP.

Goat anti Bovine MCP-1:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2367B
Datasheet
E WB
SDS
50 µg loader

Goat anti Bovine MCP-1 antibody recognizes bovine monocyte chemotactic protein-1 (MCP-1), also known as C-C motif chemokine 2 (CCL2). Bovine MCP-1 is a 99 amino acid, 11.1 kDa secreted protein.

The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.

MCP-1 plays important roles in the inflammatory response and routine immune surveillance of blood monocytes and tissue macrophages (Deshmane et al. 2009).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to bovine MCP-1 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine MCP-1
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P28291
Entrez Gene
CCL2
GO Terms
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1 ug/ml 1.5 ug/ml
Western Blotting 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection antibody in a sandwich ELISA together with AHP2366 as the capture reagent. Recombinant bovine MCP-1 (PBP024) may be used as a standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Bovine MCP-1 AHP2366 E WB 0.1 mg loader
Recombinant Bovine MCP-1 PBP024 E 5 µg loader

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