Mouse IgG2a Negative Control antibody

Mouse IgG2a Negative Control:Amethyst Orange

Product Type
Negative/Isotype Control
Isotype
IgG2a
Specificity
Mouse IgG2a Negative Control

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MCA929AMO
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Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.

Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.

Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

This antibody may not be suitable for intracellular staining on some cell types.

Target Species
Negative Control
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Activated rat T-helper cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Amethyst Orange 405 540
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

References for Mouse IgG2a Negative Control antibody

  1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
    Blood. 103 (8): 2981-9.
  2. Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
    Avian Pathol. Jan 26:1-24. [Epub ahead of print]
  3. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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