CD25 Antibody | AV142

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CD25 Antibody | AV142 gallery image 1

ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody (AV142). Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.)

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CD25 Antibody | AV142 gallery image 2

Western blot analysis of chicken CD25/huIg fusion protein with Mouse anti Chicken CD25 (AV142). Recombinant huIg was used as negative control (Image courtesy of the Roslin Institute.)

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CD25 Antibody | AV142 gallery image 3

Staining of ConA-stimulated chicken splenocytes with Mouse anti Chicken CD25 (AV142) conjugated with Alexa fluor-647
(Image courtesy of the Roslin Institute.)

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  • CD25 Antibody | AV142 thumbnail image 1
  • CD25 Antibody | AV142 thumbnail image 2
  • CD25 Antibody | AV142 thumbnail image 3
  • Mouse anti Chicken CD25
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AV142
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5925GAE, F, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5925GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Chicken CD25 monoclonal antibody, clone AV142 recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Rα) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature.
      Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
      CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection .
      Chicken IL-2Rα is the low affinity receptor for Interleukin-2.

      Mouse anti Chicken CD25 monoclonal antibody, clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad.
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Chicken CD25/huIg fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
      This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
      This product is suitable for use in direct ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified AV142 E, F, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG (H/L) (Multi Species Adsorbed)STAR1170.5 mgE, F, WB
      STAR117

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD4:FITCMCA2164F0.1 mgF
          MCA2164F
          Mouse anti Chicken CD8 Alpha:FITCMCA2166F0.1 mgF
          MCA2166F
          Human anti Chicken CD25HCA1730.1 mgF
          HCA173
          Mouse anti Chicken CD8 Alpha:RPEMCA2166PE0.1 mgF
          MCA2166PE
          Mouse anti Chicken CD4:RPEMCA2164PE100 TestsF
          MCA2164PE

          Recommended Positive Controls

            Histology Controls

              References

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