CD8 Alpha antibody | 11-39
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|Mouse anti chicken CD8 alpha, clone 11-39 recognizes the alpha chain of the chicken CD8 homologue, a 33-35 kDa cell surface protein. CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells. Mouse anti chicken CD8 alpha, clone 11-39 recognizes all polymorphic forms of chicken CD8 alpha.
Mouse anti chicken CD8 alpha, clone 11-39 has been demonstrated to cross react with Turkey (Li et al. 1999).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Turkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1.0 ml distilled water
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Chicken T-cells.
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD8 Alpha antibody
Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
Scand J Immunol. 42 (1): 171-4.
Luhtala, M. et al. (1997) Polymorphism of chicken CD8-alpha, but not CD8-beta.
Immunogenetics. 46 (5): 396-401.
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31.
McKenna, G.F. (2003) Immunopathologic investigations with an attenuated chicken anemia virus in day-old chickens.
Avian Dis. 47: 1339-45.
Morimura, T. et al. (1996) Apoptosis and CD8-down-regulation in the thymus of chickens infected with Marek's disease virus.
Arch Virol. 141 (11): 2243-9.
Luhtala M (1998) Chicken CD4, CD8alphabeta, and CD8alphaalpha T cell co-receptor molecules.
Poult Sci. 77 (12): 1858-73.
Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
J Immunol. 165 (12): 6716-22.
Arstila, T.P. & Lassila, O. (1993) Androgen-induced expression of the peripheral blood gamma delta T cell population in the chicken.
J Immunol. 151 (12): 6627-33.
View The Latest Product References
Bohls, R.L. et al. (2006) The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes.
Dev Comp Immunol. 30 (9): 843-50.
Powell, F.L. et al. (2009) The turkey, compared to the chicken, fails to mount an effective early immune response to Histomonas meleagridis in the gut.
Parasite Immunol. 31 (6): 312-27.
Katevuo, K. & Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
Immunology. 89 (3): 419-23.
Morimura, T. et al. (1995) Immunomodulation of peripheral T cells in chickens infected with Marek's disease virus: involvement in immunosuppression.
J Gen Virol. 76 ( Pt 12): 2979-85.
Powell, F. et al. (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8α and CD28.
Dev Comp Immunol. 33 (4): 540-6.
Juul-Madsen, H.R. et al. (2002) Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection.
Poult Sci. 81 (5): 649-56.
Wang, Y. et al. (2003) A novel method to analyze viral antigen-specific cytolytic activity in the chicken utilizing flow cytometry.
Vet Immunol Immunopathol. 95 (1-2): 1-9.
Arstila, T.P. et al. (1995) Primed avian γδ T cells respond to mycobacterial antigens, but show no preference for the 65-kDa heat shock protein.
Cell Immunol. 162 (1): 74-9.
Arstila, T.P. et al. (1994) γδ and αβ T cells are equally susceptible to apoptosis.
Scand J Immunol. 40 (2): 209-15.
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7.
Röhe I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73.
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-9.
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