CD8 Alpha antibody | 11-39
Mouse anti chicken CD8 alpha, clone 11-39 recognizes the alpha chain of the chicken CD8 homologue, a 33-35 kDa cell surface protein. CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells. Mouse anti chicken CD8 alpha, clone 11-39 recognizes all polymorphic forms of chicken CD8 alpha.
Mouse anti chicken CD8 alpha, clone 11-39 has been demonstrated to cross react with Turkey (Li et al. 1999).
Mouse anti chicken CD8 alpha, clone 11-39 has been demonstrated to cross react with Turkey (Li et al. 1999).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- Chicken T-cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Product Specific References
References for CD8 Alpha antibody
-
Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
Scand J Immunol. 42 (1): 171-4. -
Luhtala, M. et al. (1997) Polymorphism of chicken CD8-alpha, but not CD8-beta.
Immunogenetics. 46 (5): 396-401. -
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31. -
McKenna, G.F. (2003) Immunopathologic investigations with an attenuated chicken anemia virus in day-old chickens.
Avian Dis. 47: 1339-45. -
Morimura, T. et al. (1996) Apoptosis and CD8-down-regulation in the thymus of chickens infected with Marek's disease virus.
Arch Virol. 141 (11): 2243-9. -
Luhtala M (1998) Chicken CD4, CD8alphabeta, and CD8alphaalpha T cell co-receptor molecules.
Poult Sci. 77 (12): 1858-73. -
Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
J Immunol. 165 (12): 6716-22. -
Arstila, T.P. & Lassila, O. (1993) Androgen-induced expression of the peripheral blood gamma delta T cell population in the chicken.
J Immunol. 151 (12): 6627-33. -
Bohls, R.L. et al. (2006) The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes.
Dev Comp Immunol. 30 (9): 843-50. -
Powell, F.L. et al. (2009) The turkey, compared to the chicken, fails to mount an effective early immune response to Histomonas meleagridis in the gut.
Parasite Immunol. 31 (6): 312-27. -
Katevuo, K. & Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
Immunology. 89 (3): 419-23. -
Morimura, T. et al. (1995) Immunomodulation of peripheral T cells in chickens infected with Marek's disease virus: involvement in immunosuppression.
J Gen Virol. 76 ( Pt 12): 2979-85. -
Powell, F. et al. (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8α and CD28.
Dev Comp Immunol. 33 (4): 540-6. -
Juul-Madsen, H.R. et al. (2002) Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection.
Poult Sci. 81 (5): 649-56. -
Wang, Y. et al. (2003) A novel method to analyze viral antigen-specific cytolytic activity in the chicken utilizing flow cytometry.
Vet Immunol Immunopathol. 95 (1-2): 1-9. -
Arstila, T.P. et al. (1995) Primed avian γδ T cells respond to mycobacterial antigens, but show no preference for the 65-kDa heat shock protein.
Cell Immunol. 162 (1): 74-9. -
Arstila, T.P. et al. (1994) γδ and αβ T cells are equally susceptible to apoptosis.
Scand J Immunol. 40 (2): 209-15. -
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7. -
Röhe I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73. -
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-9.
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