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CD8 Alpha antibody | 11-39

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Anti Chicken CD8 Alpha Antibody, clone 11-39 thumbnail image 1 Anti Chicken CD8 Alpha Antibody, clone 11-39 thumbnail image 2
Anti Chicken CD8 Alpha Antibody, clone 11-39 gallery image 1
Figure A. RPE conjugated Mouse anti Chicken CD4 (MCA2164PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Chicken CD4 (MCA2164PE) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Chicken CD8 Alpha Antibody, clone 11-39 gallery image 2
Figure A. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and RPE conjugated Mouse anti Chicken CD8 (MCA2166PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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Mouse anti Chicken CD8 Alpha:RPE

Product Type
Monoclonal Antibody
Clone
11-39
Isotype
IgG1
Specificity
CD8 Alpha

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MCA2166PE
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Mouse anti chicken CD8 alpha, clone 11-39 recognizes the alpha chain of the chicken CD8 homologue, a 33-35 kDa cell surface protein. CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells. Mouse anti chicken CD8 alpha, clone 11-39 recognizes all polymorphic forms of chicken CD8 alpha.

Mouse anti chicken CD8 alpha, clone 11-39 has been demonstrated to cross react with Turkey (Li et al. 1999).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Chicken T-cells.
Fusion Partners
Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for CD8 Alpha antibody

  1. Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
    Scand J Immunol. 42 (1): 171-4.
  2. Luhtala, M. et al. (1997) Polymorphism of chicken CD8-alpha, but not CD8-beta.
    Immunogenetics. 46 (5): 396-401.
  3. Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
    Poult Sci. 78 (11): 1526-31.
  4. McKenna, G.F. (2003) Immunopathologic investigations with an attenuated chicken anemia virus in day-old chickens.
    Avian Dis. 47: 1339-45.
  5. Morimura, T. et al. (1996) Apoptosis and CD8-down-regulation in the thymus of chickens infected with Marek's disease virus.
    Arch Virol. 141 (11): 2243-9.
  6. Luhtala M (1998) Chicken CD4, CD8alphabeta, and CD8alphaalpha T cell co-receptor molecules.
    Poult Sci. 77 (12): 1858-73.
  7. Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
    J Immunol. 165 (12): 6716-22.
  8. Arstila, T.P. & Lassila, O. (1993) Androgen-induced expression of the peripheral blood gamma delta T cell population in the chicken.
    J Immunol. 151 (12): 6627-33.
    9. View The Latest Product References
  9. Bohls, R.L. et al. (2006) The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes.
    Dev Comp Immunol. 30 (9): 843-50.
  10. Powell, F.L. et al. (2009) The turkey, compared to the chicken, fails to mount an effective early immune response to Histomonas meleagridis in the gut.
    Parasite Immunol. 31 (6): 312-27.
  11. Katevuo, K. & Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
    Immunology. 89 (3): 419-23.
  12. Morimura, T. et al. (1995) Immunomodulation of peripheral T cells in chickens infected with Marek's disease virus: involvement in immunosuppression.
    J Gen Virol. 76 ( Pt 12): 2979-85.
  13. Powell, F. et al. (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8α and CD28.
    Dev Comp Immunol. 33 (4): 540-6.
  14. Juul-Madsen, H.R. et al. (2002) Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection.
    Poult Sci. 81 (5): 649-56.
  15. Wang, Y. et al. (2003) A novel method to analyze viral antigen-specific cytolytic activity in the chicken utilizing flow cytometry.
    Vet Immunol Immunopathol. 95 (1-2): 1-9.
  16. Arstila, T.P. et al. (1995) Primed avian γδ T cells respond to mycobacterial antigens, but show no preference for the 65-kDa heat shock protein.
    Cell Immunol. 162 (1): 74-9.
  17. Arstila, T.P. et al. (1994) γδ and αβ T cells are equally susceptible to apoptosis.
    Scand J Immunol. 40 (2): 209-15.
  18. Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
    Arch Virol. 159 (6): 1453-7.
  19. Röhe I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
    Poult Sci. 96 (7): 2064-73.
  20. Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
    Int J Curr Microbiol App Sci. 6 (1): 15-9.

RRID
AB_2260137
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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