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CD4 antibody | 2-35

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Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 1 Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 2 Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 3
Anti Chicken CD4 Antibody, clone 2-35 gallery image 1
Figure A. RPE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. PE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse anti Chicken CD4 (MCA2164F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Chicken CD4 Antibody, clone 2-35 gallery image 2
Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse anti Chicken CD4 (MCA2164PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Chicken CD4 Antibody, clone 2-35 gallery image 3
Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG2b isotype control (MCA691) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD4 (MCA2164GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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Mouse anti Chicken CD4:RPE

Product Type
Monoclonal Antibody
Clone
2-35
Isotype
IgG2b
Specificity
CD4

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Product Code Applications Pack Size List Price Your Price Qty
MCA2164PE
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F 100 Tests loader
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Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Chicken embryonic thymocytes
Fusion Partners
Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG2b Negative Control:RPE

References for CD4 antibody

  1. Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
    Prog Clin Biol Res. 307: 45-56.
  2. Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
    Poult Sci. 78 (11): 1526-31.
  3. Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
    J Immunol. 162 (7): 4115-21.
  4. Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
    J Gen Virol. 91 (Pt 4): 847-57.
  5. Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
    Hybridoma. 12: 633-46.
  6. Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
    Arch Virol. 159 (6): 1453-7.
  7. Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
    Avian Dis. 60 (1 Suppl): 262-8.
  8. Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
    Poult Sci. 96 (7): 2064-73.
    9. View The Latest Product References
  9. Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
    Vaccine. 33 (36): 4526-32.
  10. Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
    Int J Curr Microbiol App Sci. 6 (1): 15-19.
  11. Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
    Pathogens. 9 (10)Oct 15 [Epub ahead of print].

RRID
AB_2075649
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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