CD4 antibody | 2-35
Mouse anti Chicken CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2-35
- Isotype
- IgG2b
- Specificity
- CD4
Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998). |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Chicken embryonic thymocytes
- Fusion Partners
- Lymph node cells from immunized Balb/c mice were fused with cells of the SP2/0 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG2b Negative Control:RPE |
References for CD4 antibody
-
Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
Prog Clin Biol Res. 307: 45-56. -
Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
Hybridoma. 12: 633-46. -
Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
J Immunol. 162 (7): 4115-21. -
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31. -
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57. -
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7. -
Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
Vaccine. 33 (36): 4526-32. -
Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
Avian Dis. 60 (1 Suppl): 262-8.
View The Latest Product References
-
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-19. -
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73. -
Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
Pathogens. 9 (10): 843. -
Konieczka, P. et al. (2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
Vet Res. 53 (1): 59. -
Hohensee, L. et al. (2024) The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens.
Vet Res. 55 (1): 5. -
Song, J. et al. (2022) GPR15-C10ORF99 functional pairing initiates colonic Treg homing in amniotes.
EMBO Rep. 23 (3): e53246. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print].
- RRID
- AB_2075649
MCA2164PE
167934 INN1703If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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