CD4 antibody | 2-35



Filter by Application:
F ResetMouse anti Chicken CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2-35
- Isotype
- IgG2b
- Specificity
- CD4
Quick Links:
Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998). |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- Chicken embryonic thymocytes
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests |
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List Price | Your Price | ||||||
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Description | Mouse IgG2b Negative Control:RPE |
References for CD4 antibody
-
Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
Prog Clin Biol Res. 307: 45-56. -
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31. -
Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
J Immunol. 162 (7): 4115-21. -
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57. -
Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
Hybridoma. 12: 633-46. -
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7. -
Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
Avian Dis. 60 (1 Suppl): 262-8. -
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73.
View The Latest Product References
-
Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
Vaccine. 33 (36): 4526-32. -
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-19. -
Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
Pathogens. 9 (10)Oct 15 [Epub ahead of print].
- RRID
- AB_2075649
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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