Etanercept antibody | AbD26183_hIgG1





Human anti Etanercept:HRP
Anti-Etanercept Antibody recognizes etanercept at the region of the fusion of p75 (TNFR2) with the Fc portion of human IgG1. It binds to both free etanercept and to etanercept bound to TNF alpha, and may be used as a detection antibody in a bridging ELISA to measure total etanercept.
Human anti Etanercept
Anti-Etanercept Antibody recognizes etanercept at the region of the fusion of p75 (TNFR2) with the Fc portion of human IgG1. It binds to both free etanercept and to etanercept bound to TNF alpha, and may be used as a detection antibody in a bridging ELISA to measure total etanercept.
- Product Type
- Monoclonal Antibody
- Clone
- AbD26183_hIgG1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA279P | E | 0.1 mg |
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HCA279 | E | 0.1 mg |
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The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug in patient samples. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD25939 (HCA276) in monovalent Fab format, as the capture antibody.
Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A).
View a summary of all anti-etanercept antibodies.
Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Etanercept
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=14.8 nM by real time, label-free molecular interaction analysis on immobilized etanercept.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Enbrel is a registered trademark of Immunex Corporation
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Enbrel is a registered trademark of Immunex Corporation
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Etanercept antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA.
Protocol: PK bridging ELISA to measure total drug - ELISA
- This product may be used in a direct or indirect ELISA or as a detection antibody in a bridging ELISA together with HCA276 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Etanercept | HCA276 | E | 0.1 mg |
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Human anti Etanercept | HCA279 | E | 0.1 mg |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Etanercept | HCA276 | E | 0.1 mg |
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