Etanercept antibody | AbD25940_hIgG4_Pro
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD25940_hIgG4_Pro, HCA277P) at a concentration of 2 μg/ml in HISPEC diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with etanercept, TNFR2, Fc control or infliximab at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD25940_hIgG4_Pro, HCA277P) titrated to the given concentrations in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data for binding to etanercept and TNFR2 are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human anti etanercept (clone AbD25939, HCA276) at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of etanercept. Detection was performed using HRP conjugated Human anti etanercept (clone AbD25940_hIgG4_Pro, HCA277P) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with etanercept at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human anti etanercept (clone AbD25940_hIgG4_Pro). Detection was performed using HRP conjugated etanercept. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human TNFα at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of etanercept (0.3 μg/ml) plus increasing concentrations of Human anti etanercept (clone AbD25940_hIgG4_Pro, or clone AbD26183_hIgG1, HCA279) in the monovalent Fab format was added. Free etanercept, still capable of binding to the human TNFα coated plate, was detected using Mouse anti Human IgG (Fc) CH2 Domain:HRP (MCA647P) and QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Human anti Etanercept:HRP
Anti-Etanercept Antibody is a recombinant, inhibitory antibody in IgG4 format; it is ideal for bioanalytical assays to measure levels of etanercept and biosimilars. It is recommended for use in a PK bridging ELISA to detect free drug, and may be used as a reference standard in an ADA assay.
Human anti Etanercept
Anti-Etanercept Antibody is a recombinant, inhibitory antibody in IgG4 format; it is ideal for bioanalytical assays to measure levels of etanercept and biosimilars. It is recommended for use in a PK bridging ELISA to detect free drug, and may be used as a reference standard in an ADA assay.
- Product Type
- Monoclonal Antibody
- Clone
- AbD25940_hIgG4_Pro
- Isotype
- IgG4
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA277P | E | 0.1 mg |
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| HCA277 | E | 0.1 mg |
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Product Details
- Product Form
- Human IgG4 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
- Product Form
- Human IgG4 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=2.4 nM by real time, label-free molecular interaction analysis on immobilized etanercept
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Enbrel is a registered trademark of Immunex Corporation - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Enbrel is a registered trademark of Immunex Corporation - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
- Regulatory
- For research purposes only
Applications of Etanercept antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA.
Protocol: PK bridging ELISA to measure free drug - ELISA
- This product may be used in a direct or indirect ELISA or as a detection antibody in a bridging ELISA. Protocol: ADA bridging ELISA
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Etanercept | HCA276 | E | 0.1 mg |
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| Human anti Etanercept | HCA277 | E | 0.1 mg |
|
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Etanercept | HCA276 | E | 0.1 mg |
|
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| Human anti Etanercept:HRP | HCA277P | E | 0.1 mg |
|
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| Human anti Etanercept | HCA279 | E | 0.1 mg |
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| Human anti Etanercept:HRP | HCA279P | E | 0.1 mg |
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