Etanercept antibody | AbD25940_hIgG4_Pro

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Human anti Etanercept:HRP

Anti-Etanercept Antibody is a recombinant, inhibitory antibody in IgG4 format; it is ideal for bioanalytical assays to measure levels of etanercept and biosimilars. It is recommended for use in a PK bridging ELISA to detect free drug, and may be used as a reference standard in an ADA assay.

Human anti Etanercept

Anti-Etanercept Antibody is a recombinant, inhibitory antibody in IgG4 format; it is ideal for bioanalytical assays to measure levels of etanercept and biosimilars. It is recommended for use in a PK bridging ELISA to detect free drug, and may be used as a reference standard in an ADA assay.

Product Type
Monoclonal Antibody
Clone
AbD25940_hIgG4_Pro
Isotype
IgG4
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA277P E 0.1 mg
HCA277 E 0.1 mg
Human anti etanercept, clone AbD25940_hIgG4_Pro is an antibody that specifically recognizes etanercept as well as the extracellular ligand-binding portion of the 75 kDa (p75) human tumor necrosis factor receptor 2 (TNFR2). Clone AbD25940_hIgG4_Pro inhibits the binding of etanercept to its target tumour necrosis factor alpha (TNFα). The antibody binds to free etanercept but not to etanercept bound to its target TNFα. The antibody binds to an epitope which is also part of the TNFα binding site and can be used to measure the levels of etanercept and biosimilar products (e.g. Etacept, Intacept) in bioanalytical assays.

CloneAbD25940_hIgG4_Pro is a fully human recombinant monoclonal antibody with an IgG4 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD25939 (HCA276) in monovalent Fab format, as the capture antibody.

Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A).

View a summary of all anti-etanercept antibodies.

Product Details

Product Form
Human IgG4 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
Product Form
Human IgG4 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Etanercept
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=2.4 nM by real time, label-free molecular interaction analysis on immobilized etanercept
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Enbrel is a registered trademark of Immunex Corporation
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Enbrel is a registered trademark of Immunex Corporation
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
Regulatory
For research purposes only

Applications of Etanercept antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA.
Protocol: PK bridging ELISA to measure free drug
ELISA
This product may be used in a direct or indirect ELISA or as a detection antibody in a bridging ELISA. Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Etanercept HCA276 0.1 mg E
Human anti Etanercept HCA277 0.1 mg E
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Etanercept HCA276 0.1 mg E
Human anti Etanercept:HRP HCA277P 0.1 mg E
Human anti Etanercept HCA279 0.1 mg E
Human anti Etanercept:HRP HCA279P 0.1 mg E