VSV-G Tag Antibody

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  • Rabbit anti VSV-G Tag
  • Rabbit anti VSV-G Tag:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1228E, WBdatasheet pdfdatasheet pdf50 µg
    AHP1228
    AHP1228BE, IFdatasheet pdfdatasheet pdf50 µg
    AHP1228B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti VSV-G Tag antibody specifically recognizes recombinant proteins containing the Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag. Epitope tags are short peptide sequences that can be attached to proteins from all species, and do not affect the protein’s biochemical properties.

      VSV is a member of the Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G). G is a type I membrane glycoprotein and is responsible for viral binding to cell surface receptors and initiating the infectious process. Rabbit anti VSV-G Tag antibody has been reported as suitable for immunofluorescence. However, filamentous staining may be observed at dilutions lower than 1:200.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to VSV-G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Immunogen
      Synthetic peptide corresponding to the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/80001/32000
      Western Blotting1/2001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/30001/12000
      Immunofluorescence1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional VSV-G Tag Antibody Formats

    Formats Applications Sizes available
    VSV-G Tag Antibody : Biotin E, IF 50 µg
    VSV-G Tag Antibody : Purified E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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