Ustekinumab Antibody | AbD17827_hIgG1

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Ustekinumab Antibody | AbD17827_hIgG1 gallery image 1

ELISA demonstrating the specificity of HCA210 using various antigens as coating reagents

Antigens were coated at 5 µg/mL on a microtiter plate overnight. After washing and blocking with 5% milk powder in PBST, HRP-conjugated HCA210 (AbD17827_hIgG1; 20µL from a 2µg/mL solution in BUF049) was added. Detection was performed using the QuantaBlu™ fluorogenic peroxidase substrate

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Inhibition of ustekinumab binding to IL-23 by HCA210 (clone AbD17827 in monovalent Fab-V5Sx2 format)

IL-23 (1.0 µg/mL) was used as the coating antigen followed by a pre-incubated mixture of the anti-idiotypic antibody AbD17827 titrated into a constant amount of ustekinumab (0.3 µg/mL). Free ustekinumab still capable of binding to the plate was detected using Mouse anti Human IgG(Fc):HRP (MCA647P). Data is shown as the mean of three measurements

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Human anti ustekinumab bridging ELISA for Anti-Drug Antibody (ADA) assay development

A microtitre plate was coated over night with ustekinumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human anti ustekinumab (clone AbD17827_hIgG1). Detection was performed using HRP-conjugated ustekinumab at a concentration of 4 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements

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  • Human anti Ustekinumab:HRP
  • Human anti Ustekinumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-ustekinumab antibody is a recombinant, high affinity anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assay development for ustekinumab and biosimilars. It is recommended as the detection antibody in PK bridging ELISA format with HCA208 or HCA209 as capture antibody.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD17827_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA210PEdatasheet pdfdatasheet pdf0.1 mg
      HCA210P
      HCA210Edatasheet pdfdatasheet pdf0.1 mg
      HCA210
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti ustekinumab, clone AbD17827_hIgG1 is a is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug ustekinumab and inhibits it binding to its target. The antibody can be used to measure the levels of ustekinumab and biosimilar products in bioanalytical assays.

        Clone AbD17827_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, conjugated to HRP and in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD17829 (HCA208) or clone AbD18334 (HCA209), both in monovalent Fab format, as the capture antibody.

        Ustekinumab (branded as Stelara®) is a fully human monoclonal antibody drug (IgG1/kappa) directed against the p40 subunit of interleukin-12 and interleukin-23 and acts by blocking the binding of these two cytokines to, and preventing activation of, T-lymphocytes. The drug has been approved for treatment of moderate to severe psoriasis and is also being investigated for the treatment of psoriatic arthritis, for multiple sclerosis and sarcoidosis. Studies indicate that it may also have potential for treating severe Crohns disease.

        View a summary of all anti-ustekinumab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Ustekinumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized ustekinumab.
      • Approx. Protein Concentrations
        IgG concentration 0.1 mg/ml
        IgG concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        STELARA® is a registered trademark of Janssen Biotech, Inc.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        STELARA® is a registered trademark of Janssen Biotech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in a direct ELISA or as a detection antibody, in its HRP conjugated format, in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        Protocol: ADA bridging ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Ustekinumab Antibody Formats

      Formats Clone Applications Sizes available
      Ustekinumab Antibody : Purified AbD17827_hIgG1 E 0.1 mg
      Ustekinumab Antibody : HRP AbD17827_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti UstekinumabHCA2080.1 mgE
            HCA208
            Human anti UstekinumabHCA2090.1 mgE
            HCA209
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti UstekinumabHCA2080.1 mgE
            HCA208
            Human anti UstekinumabHCA2090.1 mgE
            HCA209
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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