IL-1 Beta Antibody

IL-1 Beta Antibody gallery image 1

Published customer image:
Rabbit anti Rat Interleukin-1β antibody used for the detection of IL-1β expressing neurons in rat brain by immnofluorescence and immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Neuronal expression of IL-1β in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1β (A) and TNF-α (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1β + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing IL-1β. (E) Immunohistochemical staining of IL-1β expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50μm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P ≤ 0.05, **P ≤ 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.

From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G.
Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
J Neuroinflammation. 2013 Sep 5;10:110

  • IL-1 Beta Antibody thumbnail image 1
  • Rabbit anti Rat Interleukin-1 beta:Biotin
  • Rabbit anti Rat Interleukin-1 beta
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAR15GE, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAR15BE, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Rat Interleukin-1 beta antibody recognizes rat IL-1 beta. Interleukin-1β is a 152 amino acid active pro-inflammatory cytokine produced with an additional 116 smino acid pro-peptide region. IL-1&beta has a broad mode of action, stimulating prostaglandin synthesis, neutrophil, T cell and B cell activation and collagen synthesis.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.09ug/ml0.14ug/ml
      Western Blotting0.1ug/ml0.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR15G as the capture reagent and PRP23 as the standard.
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AAR15B as the detection reagent and PRP23 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Beta Antibody Formats

    Formats Applications Sizes available
    IL-1 Beta Antibody : Biotin E, WB 50 µg
    IL-1 Beta Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin


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