IL-1 Beta Antibody

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IL-1 Beta Antibody gallery image 1

Published customer image:
Rabbit anti Rat Interleukin-1β antibody used for the detection of IL-1β expressing neurons in rat brain by immnofluorescence and immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Neuronal expression of IL-1β in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1β (A) and TNF-α (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1β + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing Il-1β. (E) Immunohistochemical staining of IL-1β expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50 µm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P = 0.05, **P = 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.

From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G. Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy. J Neuroinflammation. 2013 Sep 5;10:110.

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  • Rabbit anti Rat Interleukin-1 beta:Biotin
  • Rabbit anti Rat Interleukin-1 beta
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAR15GE, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAR15G
    AAR15BE, WBdatasheet pdfdatasheet pdf50 µg
    AAR15B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat Interleukin-1 beta antibody recognizes rat IL-1 beta.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml2.0ug/ml
      Functional Assays0.09ug/ml0.14ug/ml
      Western Blotting0.1ug/ml0.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR15G as the capture reagent and PRP23 as the standard.
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AAR15B as the detection reagent and PRP23 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Beta Antibody Formats

    Formats Applications Sizes available
    IL-1 Beta Antibody : Biotin E, WB 50 µg
    IL-1 Beta Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin

              References

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