Collagen I Antibody

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Collagen I Antibody gallery image 1

Published customer image:
Rabbit anti Rat collagen I antibody used for demonstration of collagen I expression in rat kidney by immunohistochemistry on paraffin embedded tissue sections.
Image caption:
Renin, collagen and PCNA stainings in renal tissue.
Representative photomicrographs of renin, collagen I and PCNA staining in the kidneys of experimental groups. Sham=sham operated controls, NMH=non-malignant hypertension, MH=malignant hypertension, neg. control=IgG control staining for specificity of antibody.

From: Hartner A, Jagusch L, Cordasic N, Amann K, Veelken R, Jacobi J and Hilgers KF (2016)
Impaired Neovascularization and Reduced Capillary Supply in the Malignant vs. Non-malignant Course of Experimental Renovascular Hypertension.
Front. Physiol. 7:370.

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  • Rabbit anti Rat Collagen I
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-1908C, E, IF, Pdatasheet pdfdatasheet pdf0.5 ml
    2150-1908
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat Collagen I antibody recognizes both native and heat denatured rat collagen type I. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.

      Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykes et al. 1990), Ehlers Danlos syndrome (Burrows et al. 1996), and idiopathic osteoporosis (Pernow et al. 2006).

      Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in Western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñoz et al. 2014).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/15001/6000
      Immunofluorescence1/101/60
      Immunohistology - Frozen1/101/60
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes.
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I Antibody Formats

    Formats Applications Sizes available
    Collagen I Antibody : Purified C, E, IF, P 0.5 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin

              References

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